Mechanisms Underlying the Virulence and Intestinal Colonization of the Vibrio alginolyticus HY9901 DctP Protein with Proteomic Analysis

IF 1 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Applied Biochemistry and Microbiology Pub Date : 2023-10-02 DOI:10.1134/S0003683823050198

Y. L. Zhang, F. Wu, Y. C. Huang, J. C. Jian, S. H. Cai

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引用次数: 1

Abstract

Vibrio alginolyticus as a common pathogen infecting humans and marine animals has become a severe threat to the global mariculture industry. Using liquid chromatography and tandem-mass spectrometry (LC–MS/MS) analysis, comparative proteomics between dctP-deletion (ΔdctP) strain and wild-type V. alginolyticus HY9901 was profiled, and total of 547 differentially expressed proteins (DEPs) were identified. In these DEPs, 310 proteins were upregulated and 237 proteins were downregulated. Comparative proteomics of ΔdctP and wild-type strains identified some important regulated factors of colonization and virulent proteins. The regulated factors of bacterial colonization mainly included LuxR and ToxR (biofilm formation), flagellins (FlaB and FlrB), mannose-sensitive hemagglutinin (MshA) pilus, various substrates of the type II secretion system (EpsD, SecD and SecF) and type IV secretion system (ClpV), quorum sensing systems (LuxR homologous ValR), the virulent heat shock proteins (IbpA and YbeY), lipopolysaccharide LptE, outer membrane proteins (OmpU and TolC), glutamate synthases (GltB and GltD), oligopeptide permease (OppA) and siderophores (IrgA). In our previous study, biological characteristics (swarming motility, biofilm formation, cell adhesion and colonized ability) were significantly decreased and LD₅₀ was significantly increased of ΔdctP compared to wide-type. Taken together, these results suggest that DctP protein plays an important role in controlling biological characteristics through DEPs in this study to finally affecting intestinal colonization and virulence.

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溶藻弧菌HY9901 DctP蛋白的毒力和肠道定殖机制的蛋白质组学分析

溶藻弧菌作为一种常见的人类和海洋动物病原体，已成为全球海水养殖业的严重威胁。使用液相色谱和串联质谱（LC–MS/MS）分析，对dctP缺失（ΔdctP）菌株和野生型溶藻弧菌HY9901之间的比较蛋白质组学进行了分析，共鉴定了547种差异表达蛋白（DEP）。在这些DEP中，310个蛋白质上调，237个蛋白质下调。ΔdctP和野生型菌株的比较蛋白质组学鉴定了一些重要的定殖调控因子和毒力蛋白。细菌定殖的调控因子主要包括LuxR和ToxR（生物膜形成）、鞭毛蛋白（FlaB和FlrB）、甘露糖敏感血凝素（MshA）菌毛、II型分泌系统（EpsD、SecD和SecF）和IV型分泌系统的各种底物、群体感应系统（LuxR同源ValR）、毒力热休克蛋白（IbpA和YbeY），脂多糖LptE、外膜蛋白（OmpU和TolC）、谷氨酸合成酶（GltB和GltD）、寡肽渗透酶（OppA）和铁载体（IrgA）。在我们之前的研究中，与宽型相比，ΔdctP的生物学特性（群集运动性、生物膜形成、细胞粘附和定植能力）显著降低，LD50显著增加。总之，这些结果表明，DctP蛋白在本研究中通过DEPs控制生物学特性，最终影响肠道定植和毒力方面发挥着重要作用。

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来源期刊

Applied Biochemistry and Microbiology 生物-生物工程与应用微生物

CiteScore

1.70

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.