[Changes of a₂-macroglobulin activity and endothelin-1 concentration in tears of rabbits after transplantation of retinal pigment epithelium cells derived from the induced pluripotent stem cells].

Q3 Biochemistry, Genetics and Molecular Biology
N V Neroeva, V V Neroev, N B Chesnokova, L A Katargina, T A Pavlenko, O V Beznos, P A Ilyukhin, O A Utkina, M A Lagarkova, P P Laktionov, A N Bogomazova, A E Kharitonov
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引用次数: 0

Abstract

Retinal diseases accompanied with the dysfunction or death of the retinal pigment epithelial (RPE) cells are widespread, hard to treat, and appear to be a leading case of visual loss and blindness among the persons older than 55 years. Transplantation of RPE cells derived from the induced pluripotent stem cells (IPSC-RPE) is a promising method of therapy for these diseases. To ensure the transplant survival instant follow-up is required. It can be based on biochemical analyses of tear fluid that can be easily non-invasively collected. For the post-transplantation process monitoring we have choosen such polyfunctional bioregulators as α2-macroglobulin (α2-MG) and endothelin-1 (ET-1). RPE atrophy in New Zealand Albino rabbits was modeled via the subretinal injection of bevacizumab. IPSC-RPE in suspension or as a monolayer on the scaffold were transplanted subretinally 1 month after the injection. α2-MG activity and ET-1 concentration in tears were estimated during the first month and after 2, 3 and 7 months after transplantation. On the 7-14 days after transplantation α2-MG activity increased in tears of the both operated and controlateral eye probably as a reaction on the corticosteroid therapy. In 50% rabbits there was one more increase after 2-3 months that could be due to the immune inflammation. Concentration of ET-1 in tears decreased dramatically on the 7-14 days and 7 months after transplantation, and it could have an influence upon the retinal vassal tone. The data obtained show that estimation of bioregulators in tears can help monitoring local metabolic processes after RPE transplantation that is necessary for the opportune, reasonable and focused medicamental correction of post-transplantation process.

[诱导多能干细胞源性视网膜色素上皮细胞移植后兔泪液中a₂-巨球蛋白活性和内皮素-1浓度的变化]。
伴随视网膜色素上皮细胞功能障碍或死亡的视网膜疾病广泛存在,难以治疗,似乎是55岁以上人群视力丧失和失明的主要病例。诱导多能干细胞(IPSC-RPE)衍生的RPE细胞移植是治疗这些疾病的一种很有前途的方法。为确保移植后存活,需要立即随访。它可以基于泪液的生化分析,可以很容易地非侵入性收集。对于移植后的过程监测,我们选择了α2-巨球蛋白(α2-MG)和内皮素-1 (ET-1)等多功能生物调节剂。通过视网膜下注射贝伐单抗建立新西兰白化兔RPE萎缩模型。注射后1个月,将IPSC-RPE悬浮液或单层支架移植到视网膜下。分别于移植后第1个月、2、3、7个月测定泪液中α2-MG活性和ET-1浓度。移植后7 ~ 14天,手术眼和对照眼泪液α2-MG活性升高,可能与皮质类固醇治疗有关。50%的兔子在2-3个月后又增加了一次,这可能是由于免疫炎症。泪液中ET-1浓度在移植后7-14天和7个月显著下降,可能对视网膜血管张力有影响。所得数据表明,泪液中生物调节因子的估计有助于监测RPE移植后局部代谢过程,这对于移植后及时、合理、集中的药物纠正是必要的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biomeditsinskaya khimiya
Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
1.30
自引率
0.00%
发文量
49
期刊介绍: The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).
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