The effect of adding carboxymethylcellulose and alginate to hyaluronic acid on reducing epidural fibrosis in a lumbar laminectomized rat model.

IF 1.1 4区医学 Q3 ORTHOPEDICS

Acta orthopaedica et traumatologica turcica Pub Date : 2022-07-01 DOI:10.5152/j.aott.2022.21392

Surachai Sae-Jung, Natthapoch Busarakham

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Abstract

Objective: This study aimed to compare the anti-epidural fibrosis and anti-inflammation effects of hyaluronic acid (HA)-carboxymethylcellulose (CMC)-alginate hydrogel, pure HA, and normal saline using a lumbar laminectomized rat model.

Methods: Thirty lumbar laminectomized adult rats were randomly assigned to three groups. The control group received normal saline, the HCA group received HA-CMC-alginate gel, and the HA group received pure HA gel soaked over the dura of the laminectomized area before closing the surgical wound. All rats were housed for eight weeks, then epidural fibrosis (EF) was histologically graded. In addition, the fibroblast and inflammatory cell density were computerized for evaluation.

Results: The mean fibroblast densities were 32.03 × 102 ± 488, 13.22 × 102 ± 200, and 14.52 × 102 ± 368 cell/mm2 in the control, HCA, and HA groups, respectively. The mean inflammatory cell density was 30.74 × 102 ± 459, 5.90 × 102 ± 129, and 11.08 × 102 ± 282 cell/mm2 in the control, HCA, and HA groups, respectively. The mean fibroblast and inflammatory cell densities in the HCA and HA groups were significantly lower than in the control group (P < 0.05). The HCA group had a significantly lower inflammatory cell density than the HA group (P < 0.05). The fibrous adherence grading of HCA and HA was significantly lower than the control (P < 0.05).

Conclusion: HA-CMC-alginate gel and HA hydrogels seem to have a better preventative effect on EF than no treatment (control). HA-CMCalginate can exhibit a better anti-inflammatory effect than HA. HA-CMC-alginate can be effective in reducing EF and inflammation after lumbar laminectomy.

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在透明质酸中加入羧甲基纤维素和海藻酸盐对腰椎椎板切除大鼠模型硬膜外纤维化的影响。

目的:本研究旨在比较透明质酸(HA)-羧甲基纤维素(CMC)-海藻酸盐水凝胶、纯透明质酸和生理盐水在腰椎椎板切除大鼠模型中的抗硬膜外纤维化和抗炎症作用。方法:将30只去椎板的成年大鼠随机分为3组。对照组给予生理盐水，HCA组给予HA- cmc -海藻酸盐凝胶，HA组在缝合手术创面前给予纯HA凝胶浸泡在层压切除区硬脑膜上。所有大鼠饲养8周，然后对硬膜外纤维化(EF)进行组织学分级。此外，计算机化成纤维细胞和炎症细胞密度进行评估。结果:对照组、HCA组和HA组的平均成纤维细胞密度分别为32.03 × 102±488、13.22 × 102±200和14.52 × 102±368个细胞/mm2。对照组、HCA组和HA组的平均炎症细胞密度分别为30.74 × 102±459、5.90 × 102±129和11.08 × 102±282个细胞/mm2。HCA组和HA组的平均成纤维细胞密度和炎症细胞密度均显著低于对照组(P < 0.05)。HCA组炎症细胞密度显著低于HA组(P < 0.05)。HCA和HA的纤维粘附等级显著低于对照组(P < 0.05)。结论:HA- cmc -海藻酸盐凝胶和HA水凝胶对EF的预防效果优于未治疗(对照组)。HA- mccalgate具有比HA更好的抗炎作用。ha - cmc -海藻酸盐可有效降低腰椎椎板切除术后EF和炎症。

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来源期刊

Acta orthopaedica et traumatologica turcica ORTHOPEDICS-

CiteScore

2.00

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Acta Orthopaedica et Traumatologica Turcica (AOTT) is an international, scientific, open access periodical published in accordance with independent, unbiased, and double-blinded peer-review principles. The journal is the official publication of the Turkish Association of Orthopaedics and Traumatology, and Turkish Society of Orthopaedics and Traumatology. It is published bimonthly in January, March, May, July, September, and November. The publication language of the journal is English. The aim of the journal is to publish original studies of the highest scientific and clinical value in orthopedics, traumatology, and related disciplines. The scope of the journal includes but not limited to diagnostic, treatment, and prevention methods related to orthopedics and traumatology. Acta Orthopaedica et Traumatologica Turcica publishes clinical and basic research articles, case reports, personal clinical and technical notes, systematic reviews and meta-analyses and letters to the Editor. Proceedings of scientific meetings are also considered for publication. The target audience of the journal includes healthcare professionals, physicians, and researchers who are interested or working in orthopedics and traumatology field, and related disciplines.