Antimicrobial effects of hydroxyapatite mosaicked polyvinyl alcohol-alginate semi-interpenetrating hydrogel-loaded with ethanolic extract of Glycyrrhiza glabra against oral pathogens.

IF 4.4 3区医学 Q2 ENGINEERING, BIOMEDICAL

Progress in Biomaterials Pub Date : 2022-12-01 Epub Date: 2022-08-15 DOI:10.1007/s40204-022-00199-2

Smitha Chenicheri, Rajesh Ramachandran, Usha Rajamanikam

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引用次数: 2

Abstract

Glycyrrhiza glabra (GG) elicits protective effects against periodontal diseases. However, the sustained bioavailability of GG extract at therapeutic concentration warrants ideal delivery vehicles. Present study has focused on the design, fabrication, and evaluations of ethanolic-crude extract of GG-loaded semi-interpenetrating network (semi-IPN) hydrogel (HAAPS-GG) using alginic acid and polyvinyl alcohol (PVA) hydrogel mosaicked with HA for periodontal regeneration. The study has examined the performance of the hydrogel against the selected oral pathogens S. mutans, E. faecalis, L. acidophilus and C. albicans. HAAPS-GG was successfully fabricated and the surface functional groups were confirmed by attenuated total reflectance-infrared (ATR-IR) spectroscopy. HAAPS-GG displayed interconnecting pores, hydrophilicity and excellent water profile contributing to the biocompatibility as evident from direct contact and MTT assay in L929 fibroblasts. The hydrogel was mechanically stable and was immunocompatible owing to the relatively decreased levels of pro-inflammatory mediators COX2, 5LPO, iNOS and MPO in RAW 264.7 macrophages. In addition, the transcript analysis on RAW 264.7 revealed the down-regulation of inflammatory transcription factor NF-κβ and the pro-inflammatory cytokine TNF-α. Importantly, HAAPS-GG arrested the progression of periodontal pathogens predominantly S. mutans, and C. albicans as evident by disc diffusion assay, MTT assay and confocal microscopy. Overall, the HAAPS-GG system offers promising translational avenues in periodontal regeneration.

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羟基磷灰石镶嵌聚乙烯醇-海藻酸盐半互穿水凝胶载甘草乙醇提取物对口腔病原菌的抗菌作用。

甘草(GG)对牙周病有保护作用。然而，GG提取物在治疗浓度下的持续生物利用度保证了理想的递送载体。本文主要研究了海藻酸和聚乙烯醇(PVA)水凝胶(HAAPS-GG)的设计、制备和评价。该研究检测了水凝胶对选定的口腔病原体变形链球菌、粪肠球菌、嗜酸乳杆菌和白色念珠菌的作用。HAAPS-GG制备成功，并通过衰减全反射-红外光谱(ATR-IR)对其表面官能团进行了确证。HAAPS-GG在L929成纤维细胞中的直接接触和MTT试验表明，HAAPS-GG具有连通孔、亲水性和良好的水谱，有助于生物相容性。由于RAW 264.7巨噬细胞中促炎介质COX2、5LPO、iNOS和MPO的水平相对降低，水凝胶具有机械稳定性和免疫相容性。此外，RAW 264.7的转录分析显示，炎症转录因子NF-κβ和促炎细胞因子TNF-α下调。重要的是，HAAPS-GG阻止了牙周病原体的进展，主要是变形链球菌和白色念珠菌，这在光盘扩散试验、MTT试验和共聚焦显微镜中得到了证实。总的来说，HAAPS-GG系统为牙周再生提供了有希望的转化途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Progress in Biomaterials MATERIALS SCIENCE, BIOMATERIALS-

CiteScore

9.60

自引率

4.10%

发文量

期刊介绍： Progress in Biomaterials is a multidisciplinary, English-language publication of original contributions and reviews concerning studies of the preparation, performance and evaluation of biomaterials; the chemical, physical, biological and mechanical behavior of materials both in vitro and in vivo in areas such as tissue engineering and regenerative medicine, drug delivery and implants where biomaterials play a significant role. Including all areas of: design; preparation; performance and evaluation of nano- and biomaterials in tissue engineering; drug delivery systems; regenerative medicine; implantable medical devices; interaction of cells/stem cells on biomaterials and related applications.