The clinical diagnostic value of plasma miR-592 and miR-217-3p levels in retinoblastoma.

IF 2 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Luo Jin Yan, Huang Bin Lin, Hu Qi Yu, Li Ru Jie, Jun Chen, Yuan Ling Mei, Yuan Peng
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引用次数: 1

Abstract

Background: This study was designed to investigate the abnormal expression of plasma miR-592 and miR-217-3p in retinoblastoma (Rb) and explore the clinical diagnostic value of their expression levels for Rb.

Methods: The 100 Rb patients who came to Nanchang Hongdu Hospital of Traditional Chinese Medicine from January 2018 to January 2019 were selected as the Rb group, and 100 healthy patients who came to the physical examination centre during the same period were selected as the control group. Real-time fluorescence quantitative PCR (qRT-PCR) was used to detect the expression levels of plasma miR-592 and miR-217-3p in all subjects; analyse the relationship between plasma miR-592 and miR-217-3p levels and the clinicopathological characteristics of Rb. Pearson correlation analysis evaluated the relationship between plasma miR-592 and miR-217-3p levels and overall survival.

Results: Plasma levels of miR-592 and miR-217-3p in the Rb group were significantly higher than those in the control group (p<0.0001), and the expression of miR-592 was significantly correlated with family genetic history (p 0.0001), tumour bias (p=0.0081), lymph node metastasis (p=0.0048) and pathological grade (p=0.0025), and the expression of miR-217-3p was significantly related to family genetic history (p 0.0001), optic nerve infiltration (p 0.0001), lymph node metastasis (p=0.0090), and pathological grade (p 0.0001). The high expression of miR-592 and miR-217-3p presents a more serious pathological manifestation of Rb, and the overall survival of patients is significantly shortened with the increase of miR-592 (r=-0.2276, p=0.0052) and miR-217-3p levels (r=-0.6461, p 0.0001).

Conclusions: and miR-217-3p are highly expressed in the plasma of Rb patients, and their elevated levels present severe pathological manifestations of Rb and shortened overall survival, which is expected to become biomarkers for clinical diagnosis of Rb.

Abstract Image

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血浆miR-592、miR-217-3p水平在视网膜母细胞瘤中的临床诊断价值。
背景:本研究旨在探讨血浆miR-592、miR-217-3p在视网膜母细胞瘤(retinoblastoma, Rb)中的异常表达,并探讨其表达水平对Rb的临床诊断价值。方法:选择2018年1月至2019年1月来南昌市洪都中医院就诊的100例Rb患者作为Rb组,同期来体检中心就诊的100例健康患者作为对照组。采用实时荧光定量PCR (Real-time fluorescence quantitative PCR, qRT-PCR)检测所有受试者血浆miR-592、miR-217-3p的表达水平;分析血浆miR-592、miR-217-3p水平与Rb临床病理特征的关系。Pearson相关分析评估血浆miR-592和miR-217-3p水平与总生存期的关系。结果:Rb组患者血浆中miR-592、miR-217-3p水平明显高于对照组(p结论:Rb患者血浆中miR-217-3p高表达,其水平升高导致Rb病理表现严重,总生存期缩短,有望成为Rb临床诊断的生物标志物。
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来源期刊
Journal of Medical Biochemistry
Journal of Medical Biochemistry BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
3.00
自引率
12.00%
发文量
60
审稿时长
>12 weeks
期刊介绍: The JOURNAL OF MEDICAL BIOCHEMISTRY (J MED BIOCHEM) is the official journal of the Society of Medical Biochemists of Serbia with international peer-review. Papers are independently reviewed by at least two reviewers selected by the Editors as Blind Peer Reviews. The Journal of Medical Biochemistry is published quarterly. The Journal publishes original scientific and specialized articles on all aspects of clinical and medical biochemistry, molecular medicine, clinical hematology and coagulation, clinical immunology and autoimmunity, clinical microbiology, virology, clinical genomics and molecular biology, genetic epidemiology, drug measurement, evaluation of diagnostic markers, new reagents and laboratory equipment, reference materials and methods, reference values, laboratory organization, automation, quality control, clinical metrology, all related scientific disciplines where chemistry, biochemistry, molecular biology and immunochemistry deal with the study of normal and pathologic processes in human beings.
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