In missed abortion the decrease of IGF-1 down-regulates PI3K/AKT signaling pathway reducing the secretion of progesterone and β-hCG

IF 1.6 4区医学 Q4 CELL BIOLOGY

Growth Hormone & Igf Research Pub Date : 2022-08-01 DOI:10.1016/j.ghir.2022.101479

Weitao Liang , Tianyuan Zhu , Na Tan , Guangzhuang Jing , Li'ao Xie , Yuhui Dang , Zhilan Li

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引用次数: 2

Abstract

Objective

To explore whether the reduction of IGF-1 in missed abortion down-regulates PI3K/AKT signaling pathway, thereby causing trophoblast cell apoptosis and reducing the secretion of β-hCG and progesterone.

Design

12 pairs of serum and villous tissues were selected from missed abortion patients and normal early pregnant women who had terminated pregnancy by artificial abortion. The subjects in two groups had same age and gestational week. Wes Simple Western system and qRT-PCR were used to detect the expression of IGF-1, IGF-1R, PI3K/AKT signaling pathway and apoptosis-related factors in villous tissues. Radioimmunoassay and Enzyme-linked immunosorbent assay were used to detect β-hCG, progesterone and IGF-1 in serum.

Results

The serum levels of β-hCG, progesterone and IGF-1 were decreased in missed abortion group than those in normal early pregnant women. In addition, compared with normal early pregnant women, the genes and proteins levels of IGF-1 and PI3K/AKT signaling pathway and anti-apoptosis related factors were significantly decreased.

Conclusions

Our results suggested that the reduction of IGF-1 in missed abortion patients could down-regulate the expression of PI3K/AKT signaling pathway, thereby increasing the apoptosis of trophoblast cells, leading to decreased secretion of β-hCG and progesterone, which may be one of the important mechanisms of missed abortion.

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在漏流产中，IGF-1的降低下调PI3K/AKT信号通路，减少孕酮和β-hCG的分泌

目的探讨漏流产中IGF-1的降低是否下调PI3K/AKT信号通路，从而引起滋养细胞凋亡，减少β-hCG和孕酮的分泌。设计选择人工流产后流产漏产和正常早孕妇女的血清和绒毛组织12对。两组受试者年龄、孕周相同。采用Wes Simple Western系统和qRT-PCR检测绒毛组织中IGF-1、IGF-1R、PI3K/AKT信号通路及凋亡相关因子的表达。采用放射免疫法和酶联免疫吸附法检测血清中β-hCG、孕酮和IGF-1。结果未流产组血清β-hCG、孕酮、IGF-1水平明显低于正常早孕组。此外，与正常早孕妇女相比，IGF-1、PI3K/AKT信号通路及抗凋亡相关因子的基因和蛋白水平均显著降低。结论IGF-1水平的降低可下调PI3K/AKT信号通路的表达，从而增加滋养细胞的凋亡，导致β-hCG和孕酮分泌减少，这可能是漏产的重要机制之一。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Growth Hormone & Igf Research 医学-内分泌学与代谢

CiteScore

3.30

自引率

0.00%

发文量

审稿时长

57 days

期刊介绍： Growth Hormone & IGF Research is a forum for research on the regulation of growth and metabolism in humans, animals, tissues and cells. It publishes articles on all aspects of growth-promoting and growth-inhibiting hormones and factors, with particular emphasis on insulin-like growth factors (IGFs) and growth hormone. This reflects the increasing importance of growth hormone and IGFs in clinical medicine and in the treatment of diseases.