Level and Duration of IgG and Neutralizing Antibodies to SARS-CoV-2 in Children with Symptomatic or Asymptomatic SARS-CoV-2 Infection.

Alka Khaitan, Dibyadyuti Datta, Caitlin Bond, Michael Goings, Katrina Co, Eliud O Odhiambo, Lucy Miller, Lin Zhang, Stephanie Beasley, Josh Poorbaugh, Chandy C John
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引用次数: 3

Abstract

There are conflicting data about level and duration of Abs to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in children after symptomatic or asymptomatic infection. In this human population, we enrolled adults and children in a prospective 6-mo study in the following categories: 1) symptomatic, SARS-CoV-2 PCR+ (SP+; children, n = 8; adults, n = 16), 2) symptomatic, PCR-, or untested (children, n = 27), 3) asymptomatic exposed (children, n = 13), and 4) asymptomatic, no known exposure (children, n = 19). Neutralizing Abs (nAbs) and IgG Abs to SARS-CoV-2 Ags and spike protein variants were measured by multiplex serological assay. All SP+ children developed nAb, whereas 81% of SP+ adults developed nAb. Decline in the presence of nAb over 6 mo was not significant in symptomatic children (100 to 87.5%; p = 0.32) in contrast to adults (81.3 to 50.0%; p = 0.03). Among children with nAb (n = 22), nAb titers and change in titers over 6 mo were similar in symptomatic and asymptomatic children. In children and adults, nAb levels postinfection were 10-fold lower than those reported after SARS-CoV-2 mRNA vaccination. Levels of IgG Abs in children to SARS-CoV-2 Ags and spike protein variants were similar to those in adults. IgG levels to primary Ags decreased over time in children and adults, but levels to three spike variants decreased only in children. Children with asymptomatic or symptomatic SARS-CoV-2 infection develop nAbs that remain present longer than in adults but wane in titer over time and broad IgG Abs that also wane in level over time. However, nAb levels were lower postinfection than those reported after immunization.

有症状或无症状的儿童SARS-CoV-2感染的IgG和中和抗体水平和持续时间
在有症状或无症状感染后的儿童中,针对严重急性呼吸综合征冠状病毒2 (SARS-CoV-2)的抗体水平和持续时间的数据存在矛盾。在这一人群中,我们招募了以下6个月的成人和儿童进行前瞻性研究:1)有症状的SARS-CoV-2 PCR+ (SP+);儿童,n = 8;成人,n = 16), 2)有症状,PCR检测或未检测(儿童,n = 27), 3)无症状暴露(儿童,n = 13), 4)无症状,未知暴露(儿童,n = 19)。采用多重血清学方法检测对SARS-CoV-2抗原和刺突蛋白变异的中和抗体(nab)和IgG抗体。所有SP+儿童发生nAb,而SP+成人发生nAb的比例为81%。在有症状的儿童中,6个月以上nAb的存在下降不显著(100 - 87.5%;P = 0.32),与成人(81.3% ~ 50.0%;P = 0.03)。在患有nAb的儿童中(n = 22),有症状和无症状儿童的nAb滴度和6个月内的滴度变化相似。在儿童和成人中,感染后nAb水平比接种SARS-CoV-2 mRNA疫苗后报告的水平低10倍。儿童对SARS-CoV-2抗原和刺突蛋白变体的IgG抗体水平与成人相似。在儿童和成人中,针对原发Ags的IgG水平随着时间的推移而下降,但针对三种尖峰变异的IgG水平仅在儿童中下降。无症状或有症状的SARS-CoV-2感染的儿童产生的抗体存在时间比成人长,但随着时间的推移滴度下降,而广泛的IgG抗体水平也随着时间的推移而下降。然而,感染后nAb水平低于免疫后报告的水平。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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