Shifts in uterine bacterial communities associated with endogenous progesterone and 17β-estradiol concentrations in beef cattle

Abstract

The relation between circulating concentrations of progesterone and 17β-estradiol prior to insemination play a key role in optimizing fertility in cattle. This study aimed to determine the impact of endogenous progesterone (P₄) and estradiol (E₂) concentrations on uterine bacterial community abundance and diversity in beef cattle. Angus-influenced heifers were subjected to an industry standard estrous synchronization protocol. Uterine flushes were collected on d -2 (endogenous P₄) and d 0 (endogenous E₂) and used for targeting the V4 hypervariable region of 16S rRNA bacterial gene. Plasma was collected on d -2 and 0 for quantification of P₄ and E₂ concentrations by radioimmunoassay, respectively. Heifers were allotted to one of the following groups: High P₄ + High E₂ (H-H; n = 11), High P₄ + Low E₂ (H-L; n = 9), Low P₄ + High E₂ (L-H; n = 9), Low P₄ + Low E₂ (L-L; n = 11). Results indicated that Shannon's diversity index tended to be greater for H-L heifers compared to L-H heifers on d 0 (P = 0.10). For H-L heifers from d -2 to d 0, the relative abundance of Actinobacteria decreased and Tenericutes increased (P < 0.01). Within phylum Actinobacteria, the relative abundance of Corynebacterium decreased from d -2 to d 0 in treatment groups H-H, H-L, and L-L (P < 0.05); however, did not differ by d for L-H heifers. Within phylum Tenericutes, the relative abundance of Ureaplasma increased from d -2 to d 0 for H-L heifers (P = 0.01). Additionally for H-L heifers, the relative abundance of Bacteroidetes tended to increase from day -2 to on d 0 (P = 0.07). For H-L heifers, uterine pH increased from day -2 to d 0 (P = 0.05). These results suggest that differing endogenous concentrations of P₄ and E₂ may be associated with shifts in uterine microbiota and pH, and this could ultimately impact fertility outcomes in beef cattle.