Trifolium pratense extract increases testosterone and improves sperm characteristics and antioxidant status in diabetic rats.

IF 1.6 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnic & Histochemistry Pub Date : 2022-11-01 Epub Date: 2022-02-14 DOI:10.1080/10520295.2022.2039766

Mohammad Rasool Khazaei, Elham Gravandi, Elham Ghanbari, Elham Niromand, Mozafar Khazaei

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引用次数: 4

Abstract

Male reproductive dysfunction is a common complication of diabetes mellitus. Trifolium pratense exhibits antioxidant and antidiabetic effects. We investigated the effects of an extract of T. pratense on serum antioxidant status, sperm characteristics, testicular tissue changes and testosterone level in diabetic rats. Male Wistar rats were divided into six groups: 1, untreated control; 2, diabetic; 3 and 4, 100 or 200 mg/kg T. pratense extract treated, respectively; 5 and 6, diabetic 100 or 200 mg/kg T. pratense extract treated, respectively. Diabetes was induced by intraperitoneal injection of streptozotocin. After 3 weeks, serum glucose, testosterone and nitric oxide (NO); sperm parameters; testicular histology and total antioxidant capacity (TAC) were evaluated. In diabetic rats treated with T. pratense extract, sperm motility, count and viability, as well as TAC and testosterone were increased significantly compared to untreated diabetic rats, while serum NO and bcl-2 and p53 expression was decreased significantly compared to untreated diabetic rats. T. pratense extract reduced testicular tissue destruction caused by diabetes.

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三叶草提取物提高糖尿病大鼠睾酮水平，改善精子特征和抗氧化状态。

男性生殖功能障碍是糖尿病的常见并发症。三叶草具有抗氧化和抗糖尿病作用。本文研究了普氏藤提取物对糖尿病大鼠血清抗氧化状态、精子特征、睾丸组织变化及睾酮水平的影响。雄性Wistar大鼠分为6组:1、对照组;2、糖尿病;分别处理3、4、100、200 mg/kg茴香提取物;5、6、糖尿病患者分别用100或200 mg/kg普氏藤提取物处理。腹腔注射链脲佐菌素诱导糖尿病。3周后，血清葡萄糖、睾酮、一氧化氮(NO);精子参数;评估睾丸组织学和总抗氧化能力(TAC)。与未处理的糖尿病大鼠相比，经pratense提取物处理的糖尿病大鼠精子活力、精子计数和活力以及TAC和睾酮均显著增加，血清NO和bcl-2、p53表达均显著降低。白藤提取物减少糖尿病引起的睾丸组织破坏。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnic & Histochemistry 生物-生物工程与应用微生物

CiteScore

3.40

自引率

6.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.