{"title":"AlliGator: A Phasor Computational Platform for Fast <i>in vivo</i> Lifetime Analysis.","authors":"Sez-Jade Chen, Nattawut Sinsuebphon, Margarida Barroso, Xavier Intes, Xavier Michalet","doi":"10.1364/omp.2017.omtu2d.2","DOIUrl":null,"url":null,"abstract":"<p><p>A fast time-gated phasor analysis tool was developed and used to monitor kinetics of NIR FLIM-FRET <i>in vitro</i> and <i>in vivo</i>. The results were validated by comparison with standard two-component fluorescence decay fitting analysis.</p>","PeriodicalId":74365,"journal":{"name":"Optical molecular probes, imaging and drug delivery","volume":"2017 ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8772506/pdf/nihms-1693534.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Optical molecular probes, imaging and drug delivery","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1364/omp.2017.omtu2d.2","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
A fast time-gated phasor analysis tool was developed and used to monitor kinetics of NIR FLIM-FRET in vitro and in vivo. The results were validated by comparison with standard two-component fluorescence decay fitting analysis.