Differential expression patterns of AIP, UCKL1, and PKN1 genes in breast cancer of different molecular subtypes.

Q3 Medicine

Experimental oncology Pub Date : 2021-12-01 DOI:10.32471/exp-oncology.2312-8852.vol-43-no-4.17067

L Kovalevska, E Kashuba, T Zadvornyj, K Astrid, N Lukianova, V Chekhun

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引用次数: 1

Abstract

Background: Classification of breast cancer (BC) in the molecular subtypes had the enormous impact on the development of the individualized therapy. Nevertheless, there is a need for additional biomarkers that would help to refine molecular subtypes of BC and propose the therapeutic approach for each patient.

Aim: To study differential expression patterns of AIP, UCKL1, and PKN1 genes in blood sera and tumor tissue of patients with BC of different molecular subtypes.

Materials and methods: The total extracellular RNA was isolated from serum of 26 BC patients. cDNAs was synthesized and quantitative polymerase chain reaction was performed. Also, immunohistochemical studies of UCKL, AIP and PKN1 were performed on deparaffined tissue sections. The study was supplemented by a bioinformatic analysis of the publicly available databases.

Results: AIP and UCKL-1 extracellular mRNA levels were 100-1000-fold increased in blood sera of all BC patients, compared to the healthy donors. The highest levels were detected in the luminal A and HER2 (ERRB2) BC subtypes. The highest levels of PKN1 were detected blood sera of the patients with luminal B and basal subtypes; its expression levels were just 10-100-fold higher in BC samples compared to healthy donors.

Conclusions: The UCKL1, AIP, PKN1 genes are overexpressed at the mRNA level in blood sera of BC patients compared to the sera of healthy individuals. Among three genes under study, only for the AIP gene, the pattern of extracellular mRNA expression in sera paralleled to protein expression in BC tissues of each specified molecular subtype.

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AIP、UCKL1和PKN1基因在不同分子亚型乳腺癌中的差异表达模式

背景:乳腺癌分子亚型的分类对个体化治疗的发展有着巨大的影响。尽管如此，仍需要额外的生物标志物来帮助改进BC的分子亚型，并为每位患者提出治疗方法。目的:研究AIP、UCKL1和PKN1基因在不同分子亚型BC患者血清和肿瘤组织中的差异表达模式。材料和方法:从26例BC患者血清中分离总细胞外RNA。合成cdna并进行定量聚合酶链反应。在离体组织切片上进行UCKL、AIP和PKN1的免疫组化研究。这项研究还得到了对公开数据库的生物信息学分析的补充。结果:与健康供者相比，所有BC患者血清中AIP和UCKL-1细胞外mRNA水平升高100-1000倍。在luminal A和HER2 (ERRB2) BC亚型中检测到最高水平。PKN1在luminal B和basal亚型患者血清中含量最高;与健康供体相比，BC样本中的表达水平仅高出10-100倍。结论:与健康人相比，BC患者血清中UCKL1、AIP、PKN1基因mRNA水平过表达。在研究的三个基因中，只有AIP基因在血清中细胞外mRNA的表达模式与各指定分子亚型BC组织中的蛋白表达模式平行。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Experimental oncology Medicine-Oncology

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： The Experimental Oncology is an English-language journal that publishes review articles, original contributions, short communications, case reports and technical advances presenting new data in the field of experimental and fundamental oncology. Manuscripts should be written in English, contain original work, which has not been published or submitted for publication elsewhere. It also implies the transfer of the Copyright from the author to “Experimental Oncology”. No part of journal publications may be reproduced, stored in a retrieval system or transmitted in any form or by any means without the prior permission of the publisher.