LncRNA FBXL19-AS1 promotes proliferation and metastasis of cervical cancer through upregulating COL1A1 as a sponge of miR-193a-5p.

IF 1.9 3区生物学 Q2 BIOLOGY

Journal of Biological Research-Thessaloniki Pub Date : 2021-08-16 DOI:10.1186/s40709-021-00151-8

Xiaoyong Huang, Haiyan Shi, Xinghai Shi, Xuemei Jiang

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引用次数: 9

Abstract

Background: Cervical cancer (CC) is one of the most common and malignant tumors in women. In this study, we aim to explore the role and mechanism of F-box and leucine rich repeat protein 19 antisense RNA 1 (FBXL19-AS1), a novel long-chain non coding RNA (lncRNA) with marked roles in a variety of tumors, in regulating the proliferation and metastasis of CC.

Methods: The expression of FBXL19-AS1, miR-193a-5p and COL1A1 were detected by RT-PCR and western blot. Gain- and loss-of functional assays of FBXL19-AS1 and miR-193a-5p were performed in CC cell lines in vitro or in vivo. The proliferation, migration, invasion, apoptosis and epithelial-mesenchymal transition (EMT) of CC cells were determined.

Results: FBXL19-AS1 and COL1A1 were significantly up-regulated in CC tissues, while miR-193a-5p was significantly down-regulated. Overexpression of FBXL19-AS1 significantly promoted the proliferation, migration, invasion, EMT and growth of CC cells and inhibited apoptosis, while knockdown of FBXL19-AS1 had the opposite effects. On the other hand, miR-193a-5p inhibited the proliferation and metastasis of CC cells. Mechanistically, FBXL19-AS1 functioned as a competitive endogenous RNA (ceRNA) and inhibited the expression of miR-193a-5p, which targeted at the 3'-UTR site of COL1A1 and negatively regulated COL1A1 expression.

Conclusions: FBXL19-AS1 promotes the proliferation and metastasis of CC cells by sponging miR-193a-5p and up-regulating COL1A1.

Abstract Image

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LncRNA FBXL19-AS1作为miR-193a-5p的海绵，通过上调COL1A1促进宫颈癌的增殖和转移。

背景:宫颈癌是女性最常见的恶性肿瘤之一。本研究旨在探讨F-box和亮氨酸富重复蛋白19反义RNA 1 (FBXL19-AS1)在多种肿瘤中具有显著作用的新型长链非编码RNA (lncRNA)在cc增殖转移中的作用及机制。方法:采用RT-PCR和western blot检测FBXL19-AS1、miR-193a-5p和COL1A1的表达。在体外或体内CC细胞系中进行FBXL19-AS1和miR-193a-5p的功能增益和丧失测定。测定CC细胞的增殖、迁移、侵袭、凋亡和上皮间质转化(EMT)。结果:FBXL19-AS1和COL1A1在CC组织中显著上调，miR-193a-5p显著下调。过表达FBXL19-AS1可显著促进CC细胞的增殖、迁移、侵袭、EMT和生长，抑制凋亡，而过表达FBXL19-AS1则相反。另一方面，miR-193a-5p抑制CC细胞的增殖和转移。在机制上，FBXL19-AS1作为竞争性内源性RNA (ceRNA)，抑制miR-193a-5p的表达，miR-193a-5p靶向COL1A1的3'-UTR位点，负向调节COL1A1的表达。结论:FBXL19-AS1通过海绵化miR-193a-5p，上调COL1A1，促进CC细胞增殖转移。

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来源期刊

Journal of Biological Research-Thessaloniki 生物-生物学

CiteScore

5.20

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Journal of Biological Research-Thessaloniki is a peer-reviewed, open access, international journal that publishes articles providing novel insights into the major fields of biology. Topics covered in Journal of Biological Research-Thessaloniki include, but are not limited to: molecular biology, cytology, genetics, evolutionary biology, morphology, development and differentiation, taxonomy, bioinformatics, physiology, marine biology, behaviour, ecology and conservation.