Determination of Required HLB Values for Citrus unshiu Fruit Oil, Citrus unshiu Peel Oil, Horse Fat and Camellia japonica Seed Oil.

Abstract

In the present study, the required hydrophilic lipophilic balance (HLB) values of Citrus unshiu fruit oil (CUFO), Citrus unshiu peel oil (CUPO), horse fat (HF), and Camellia japonica seed oil were determined empirically by preparing oil-in-water (o/w) emulsions. Lipophilic and hydrophilic surfactants were prepared in various ratios in o/w emulsion. The droplet size of the emulsion was measured using a particle size analyzer, and the turbidity was measured using a turbidity meter and a ultraviolet (UV)-vis spectrophotometer. According to the Orafidiya-Oladimeji method, the HLB value of the emulsion having the minimum dispersion ratio, the minimum droplet size, and the maximum turbidity degree was determined as the required HLB value for each essential oil. Based on these methods, the required HLB values of CUFO, CUPO, HF, and Camellia japonica seed oil were determined as 14.75-14.90, 15.35-15.40, 6.30-7.06, and 5.94-6.30, respectively.