Haplotype-based association study between PRCP gene polymorphisms and essential hypertension in Hani minority group from a remote region of China.

IF 2.1 4区医学 Q3 PERIPHERAL VASCULAR DISEASE

Journal of the Renin-Angiotensin-Aldosterone System Pub Date : 2020-10-01 DOI:10.1177/1470320320981316

Yanrui Wu, Xingming Pan, Xiaoxiao Jin

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引用次数: 0

Abstract

Objective: Prolylcarboxypeptidase (PRCP) is both involved in the Kallikrein-Kinin system (KKS) and renin-angiotensin-aldosterone system (RAAS). This study aimed to determine the genetic impact of PRCP gene polymorphisms on essential hypertension (EH) in an isolated population from a remote region of China.

Methods: A haplotype-based study was investigated in 346 EH patients and 346 normal subjects and all samples were Hani minority residents in Southwest China. A total of 11 tag single nucleotide polymorphisms (SNPs) in PRCP gene were tested by polymerase chain reaction-restriction fragment length polymorphism method.

Results: Single site analysis found that PRCP gene 3'UTR SNP rs3750931 was associated with EH. The minor allele G of rs3750931 was more prevalent in the EH patients compared to control subjects after Bonferroni correction (p < 0.05). Moreover, the rs3750931 G allele carriers showed higher average blood pressure (BP) level among the subjects. The H2 (GAGCACTAACA) haplotype without rs3750931 G allele showed the protective effect for EH (OR = 0.68, 95 CI 0.54-0.85, p = 0.001).

Conclusion: The present study indicated PRCP gene rs3750931 was associated with the risk of EH. This SNP G allele could be considered as one of risk markers for EH in Hani population.

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中国偏远地区哈尼族人群PRCP基因多态性与原发性高血压的单倍型相关性研究

目的:prolycarboxypeptidase (PRCP)是一种既参与钾likrein- kinin系统(KKS)又参与肾素-血管紧张素-醛固酮系统(RAAS)的酶。本研究旨在确定PRCP基因多态性对中国偏远地区孤立人群原发性高血压(EH)的遗传影响。方法:对346例EH患者和346例正常人进行单倍型研究，样本均为西南地区哈尼族居民。采用聚合酶链反应-限制性片段长度多态性法检测了PRCP基因11个标签单核苷酸多态性(snp)。结果:单位点分析发现PRCP基因3'UTR SNP rs3750931与EH相关。经Bonferroni校正后，EH患者中rs3750931的小等位基因G比对照组更普遍(p p = 0.001)。结论:PRCP基因rs3750931与EH发生风险相关。该SNP G等位基因可作为哈尼族人群EH的危险标志之一。

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来源期刊

Journal of the Renin-Angiotensin-Aldosterone System 医学-外周血管病

CiteScore

6.20

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： JRAAS is a peer-reviewed, open access journal, serving as a resource for biomedical professionals, primarily with an active interest in the renin-angiotensin-aldosterone system in humans and other mammals. It publishes original research and reviews on the normal and abnormal function of this system and its pharmacology and therapeutics, mostly in a cardiovascular context but including research in all areas where this system is present, including the brain, lungs and gastro-intestinal tract.