Automated Two-Chamber Operon ID/ED Task for Mice

Q2 Neuroscience

Current Protocols in Neuroscience Pub Date : 2020-12-04 DOI:10.1002/cpns.109

Francesca Scarsi, Diego Scheggia, Francesco Papaleo

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引用次数: 3

Abstract

Attentional set shifting is a measure of cognitive flexibility and executive functions widely assessed in humans by the Wisconsin Card Sorting Test (WCST) and the CANTAB Intra‐/Extra‐Dimensional set‐shifting task (ID/ED). The recently established automated two‐chamber “Operon ID/ED” task for mice has proved to be an effective preclinical tool for drug testing and genetic screening, with direct translational valence in healthy human subjects and patients with schizophrenia. Here, we describe an upgraded version of the Operon ID/ED task that is now commercially available. This automated task allows one to study the ability of mice to shift attention through different rules, using two or three different dimensions (i.e., lights, odors, and textures). This unit provides a detailed step‐by‐step protocol for preparing and testing the mice that includes all procedures required for this upgraded attentional set‐shifting paradigm. A short manual for the use of the dedicated ANY‐maze software and tools for adapting it to different needs are also provided. Overall, this is a comprehensive guideline for the use of this complex upgraded equipment and paradigm. © 2020 Wiley Periodicals LLC.

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小鼠自动双腔操作子ID/ED任务

注意集转移是一种认知灵活性和执行功能的测量方法，被威斯康星卡片分类测验(WCST)和CANTAB内/外维集转移任务(ID/ED)广泛评估。最近建立的小鼠自动双腔“操纵子ID/ED”任务已被证明是药物测试和基因筛选的有效临床前工具，在健康人类受试者和精神分裂症患者中具有直接的翻译价。在这里，我们描述了一个升级版本的Operon ID/ED任务，它现在是商业上可用的。这项自动化任务允许人们研究老鼠通过不同规则转移注意力的能力，使用两到三个不同的维度(即光线、气味和纹理)。本单元为准备和测试小鼠提供了详细的一步一步的协议，包括该升级的注意力集转移范式所需的所有程序。还提供了一份使用专用ANY-maze软件的简短手册，以及使其适应不同需求的工具。总的来说，这是使用这种复杂的升级设备和范例的综合指南。©2020 Wiley期刊有限公司基本协议:Operon ID/ED测试支持协议:使用ANY-maze软件

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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期刊介绍： Current Protocols in Neuroscience is a one-stop resource for finding and adapting the best models and methods for all types of neuroscience experiments. Updated every three months in all formats, CPNS is constantly evolving to keep pace with the very latest discoveries and developments. A year of these quarterly updates is included in the initial CPNS purchase price.