Oxidant and antioxidant balance in children with bacteremia.

IF 2.6 4区医学 Q2 Medicine

Minerva pediatrica Pub Date : 2023-12-01 Epub Date: 2020-09-03 DOI:10.23736/S2724-5276.20.05748-5

Kubra Aykac, Yasemin Ozsurekci, Sevgen Tanir Basaranoglu, Osman O Demir, Gamze Avcioglu, Ozcan Erel, Mehmet Ceyhan

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引用次数: 0

Abstract

Background: There is a crucial balance between oxidant and antioxidant defense mechanisms. We aimed to evaluate the role of the balance of these systems in children with bloodstream infection.

Methods: We analyzed prospectively oxidant and antioxidant stress parameters from serum samples of children with BSI besides demographic and clinical data of children. Serum levels of the total antioxidant status (TAS), total oxidant status (TOS), albumin, plasma thiol, disulphide, catalase (CAT), myeloperoxidase (MPO), ischemia-modified albumin (IMA) levels, ferroxidase and arylesterase (ARES) activity were evaluated in both patients and healthy controls.

Results: A total of 113 children were evaluated, 50 of them had bacteremia and the remaining 63 were healthy subjects. The median TOS values were 18.5 µmol H2O2/L and 13.1 µmol H2O2/L in patient and control groups, respectively with a statistically significant difference between groups. The mean serum IMA levels were 0.8±0.1 absorbance unit (ABSU) in patients and 0.5±0.09 ABSU in control, the difference between groups was statistically significant. The native thiol, total thiol levels and the disulphide levels were significantly lower in the patient group as compared with the control group. The myeloperoxidase level was 136 U/L in patients and 107 in controls with a statistically significant difference between groups.

Conclusions: TOS, IMA, MPO, and particularly plasma thiols seem good candidates for accurate diagnosis of bacteremia in children.

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菌血症患儿的氧化剂和抗氧化剂平衡。

背景:在氧化和抗氧化防御机制之间有一个至关重要的平衡。我们的目的是评估这些系统的平衡在儿童血液感染中的作用。方法:对BSI患儿血清中氧化应激和抗氧化应激参数进行前瞻性分析。评估两组患者和健康对照者的血清总抗氧化状态(TAS)、总氧化状态(TOS)、白蛋白、血浆硫醇、二硫、过氧化氢酶(CAT)、髓过氧化物酶(MPO)、缺血修饰白蛋白(IMA)水平、氧化铁酶和芳香酯酶(ARES)活性。结果:共评估儿童113例，其中菌血症50例，健康儿童63例。患者和对照组的TOS中位值分别为18.5µmol H2O2/L和13.1µmol H2O2/L，组间差异有统计学意义。患者平均血清IMA水平为0.8±0.1 ABSU，对照组为0.5±0.09 ABSU，组间差异有统计学意义。与对照组相比，患者组的天然硫醇、总硫醇和二硫化物水平明显降低。患者髓过氧化物酶水平为136 U/L，对照组为107 U/L，组间差异有统计学意义。结论:TOS, IMA, MPO，特别是血浆硫醇似乎是准确诊断儿童菌血症的良好候选者。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Minerva pediatrica PEDIATRICS-

CiteScore

2.70

自引率

3.80%

发文量

审稿时长

>12 weeks

期刊介绍： Minerva Pediatrica publishes scientific papers on pediatrics, neonatology, adolescent medicine, child and adolescent psychiatry and pediatric surgery. Manuscripts may be submitted in the form of editorials, original articles, review articles, special articles, letters to the Editor and guidelines. The journal aims to provide its readers with papers of the highest quality and impact through a process of careful peer review and editorial work.