Reproductive toxicity assessment of Olusosun municipal landfill leachate in Mus musculus using abnormal sperm morphology and dominant lethal mutation assays.

Environmental analysis, health and toxicology Pub Date : 2020-06-01 Epub Date: 2020-06-30 DOI:10.5620/eaht.e2020010
Olaoluwa J Ademola, Chibuisi G Alimba, Adekunle A Bakare
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引用次数: 7

Abstract

Mixture of xenobiotics in Olusosun landfill leachates (OSL) induced somatic and germ cell genotoxicity in eukaryotic cells. However, whether the DNA-damaged germ cells can increase reproductive failure, embryotoxicity and/or teratogenicity during fertilization remained unclear. The study herein investigated reproductive toxicity and embryotoxic effects of OSL in mice using abnormal sperm morphology and dominant lethal mutation assays. Six mice per group were exposed to 0.5 mL of 5, 10, 25, and 50% OSL (v/v, leachate/distilled water) for 5 consecutive days. Similar treatment was giving to distilled water and cyclophosphamide (20 mg/kg body weight), as negative and positive control groups, respectively. At 35 day post-treatment, the mice were sacrificed and examined for cauda epididymal abnormal sperm morphology, testicular weight change and histopathological lesions. Male mice were mated to untreated nulliparous females (1 treated male per 2 untreated females) until vaginal plugs were observed. Pregnant females were sacrificed on 14th day post-mating and uterine content examined for number of implants per female, resorptions, and live and dead fetuses. OSL significantly increased teratozoospermia by 31.02% with amorphous sperm head having the highest frequency and sperm with two heads, the least. Histology of the testes revealed congestion of the interstitial blood vessels, spermatid retention and disorganization of the germinal epithelium from the basal compartment. OSL insignificantly increased absolute and relative testes weights but reduced body weight of exposed mice. There was insignificant induction of dominant lethal mutation, although low fertility was observed in the treated mice. The analyzed physico-chemical parameters and heavy metals/metalloids in OSL were higher than permissible limits. The metals, physico-chemical parameters and other unanalyzed substances in OSL induced abnormal sperm morphology, altered testes pathology, but did not significantly induce dominant lethal mutation in mice. Constituents of OSL are toxic to the male reproductive organs in mice and suggests harmful impact on public health. Indiscriminate disposal of solid wastes in the environment should be prevented.

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用异常精子形态和显性致死突变法评价奥鲁索松城市垃圾渗滤液对小家鼠的生殖毒性。
奥鲁索孙垃圾渗滤液(OSL)中混合外源生物诱导真核细胞的体细胞和生殖细胞遗传毒性。然而,dna受损的生殖细胞是否会增加受精过程中的生殖失败、胚胎毒性和/或致畸性尚不清楚。本研究采用精子形态异常和显性致死突变的方法研究了OSL对小鼠的生殖毒性和胚胎毒性作用。每组6只小鼠连续5天暴露于0.5 mL 5、10、25和50% OSL (v/v,渗滤液/蒸馏水)。将蒸馏水和环磷酰胺(20 mg/kg体重)分别作为阴性对照组和阳性对照组进行类似处理。治疗后35 d处死小鼠,检查附睾尾精子形态异常、睾丸重量变化及组织病理学病变。雄鼠与未治疗的未生育雌鼠交配(1只治疗的雄鼠与2只未治疗的雌鼠交配),直到观察到阴道堵塞。孕母在交配后第14天处死,检查子宫内容物的数量,每个女性的植入物数量,再吸收,活胎和死胎。OSL显著增加畸形精子症的发生率为31.02%,其中无定形精子头发生率最高,双头精子发生率最低。睾丸组织学显示间质血管充血,精细胞滞留,基底室生发上皮组织紊乱。OSL对小鼠睾丸绝对重量和相对重量的影响不显著,但对小鼠体重的影响不显著。虽然在治疗小鼠中观察到低生育能力,但没有显著的显性致死突变诱导。分析的OSL理化参数和重金属/类金属均高于允许限量。OSL中金属、理化参数等未分析物质可引起小鼠精子形态异常、睾丸病理改变,但未引起小鼠显性致死突变。OSL的成分对小鼠的雄性生殖器官有毒性,并提示对公众健康的有害影响。应防止在环境中随意处置固体废物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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