Comparative Evaluation of Bone Repair with Four Different Bone Substitutes in Critical Size Defects.

IF 3 Q3 MATERIALS SCIENCE, BIOMATERIALS
International Journal of Biomaterials Pub Date : 2020-05-23 eCollection Date: 2020-01-01 DOI:10.1155/2020/5182845
Gustavo Grossi-Oliveira, Leonardo P Faverani, Bruno Coelho Mendes, Tárik Ocon Braga Polo, Gabriel Cury Batista Mendes, Valthierre Nunes de Lima, Paulo Domingos Ribeiro Júnior, Roberta Okamoto, Osvaldo Magro-Filho
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引用次数: 3

Abstract

This study evaluated the osteoconductive potential of four biomaterials used to fill bone defects. For this, 24 male Albino rabbits were submitted to the creation of a bilateral 8 mm calvarial bone defect. The animals were divided into four groups-bovine hydroxyapatite, Bio-Oss® (BIO); Lumina-Bone Porous® (LBP); Bonefill® (BFL); and an alloplastic material, Clonos® (CLN)-and were euthanized at 14 and 40 days. The samples were subjected to histological and histometric analysis for newly formed bone area. Immunohistochemical analysis for Runt-related transcription factor 2 (Runx2), vascular endothelial growth factor (VEGF), and osteocalcin (OC) was performed. After statistical analysis, the CLN group showed greater new bone formation (NB) in both periods analyzed (p < 0.05). At 14 days, the NB showed greater values in BIO in relation to LBP and BFL groups; however, after 40 days, the LBP group surpassed the results of BIO (p < 0.001). The immunostaining showed a decrease in Runx2 intensity in BIO after 40 days, while it increased for LBP (p < 0.05). The CLN showed increased OC compared to the other groups in both periods analyzed (p < 0.05). Therefore, CLN showed the best osteoconductive behavior in critical defects in rabbit calvaria, and BFL showed the lowest osteoconductive property.

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四种不同骨替代物修复临界尺寸骨缺损的比较评价。
本研究评估了四种用于骨缺损填充的生物材料的骨传导潜能。为此,24只雄性白化兔被提交到双侧8毫米颅骨骨缺损的创建。动物分为四组:牛羟基磷灰石,BIO - oss®(BIO);Lumina-Bone Porous®(LBP);Bonefill®(BFL);和同种异体材料Clonos®(CLN),并在第14天和第40天实施安乐死。对新生骨区进行组织学和组织计量学分析。对runt相关转录因子2 (Runx2)、血管内皮生长因子(VEGF)和骨钙素(OC)进行免疫组化分析。经统计学分析,两期CLN组新骨形成(NB)均高于对照组(p < 0.05)。在第14天,与LBP和BFL组相比,BIO组的NB值更高;然而,40天后,LBP组优于BIO组(p < 0.001)。免疫染色显示,40天后BIO组Runx2强度降低,LBP组Runx2强度升高(p < 0.05)。与其他组相比,CLN组在两个时间段均显示OC升高(p < 0.05)。因此,CLN在兔颅骨关键缺损中表现出最好的骨导电性,BFL表现出最低的骨导电性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
International Journal of Biomaterials
International Journal of Biomaterials MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
4.30
自引率
3.20%
发文量
50
审稿时长
21 weeks
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