Wancong Zhang , Hanxing Zhao , Jiasheng Chen , Xiaoping Zhong , Weiping Zeng , Bingna Zhang , Kai Qi , Zhonglei Li , Jianda Zhou , Lungang Shi , Zhihao He , Shijie Tang
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引用次数: 4
Abstract
Background
Recent studies have shown that lipid metabolism was abnormal during the formation of cleft palate. However, the composition of these lipid species remains unclear.
Objective
Aims of this study were to identify the lipid species components and reveal the key lipid metabolic disorders in cleft palate formation.
Methods
The pregnant mice were divided into experimental group exposed to all-trans retinoic acid (RA-treated group) (n = 12) and control group (n = 12) at embryonic gestation day 10.5 (E0.5). The component of the palatal tissue metabolome was analyzed using a LCMS-based nontargeted lipidomics approach. Multivariate statistical analysis was then carried out to assess the differences between the RA-treated group and the control group.
Results
Twenty-nine lipid species were found to discriminate between RA-treated and control embryos. Among them, 28 lipid species increased and 1 lipid species decreased in the RA-treated group. Among these lipids, 13 were triglycerides, 9 were PEs, 3 were PCs, 2 were PSs, 2 were DGs. Further analysis of the number of carbons and unsaturated bond of triglycerides showed that TGs with high unsaturated bonds constituted a higher fraction in the RA-treated group. A higher amount of triglycerides containing 52, 54, 56, 58, 60 carbons, and 1 to 8 unsaturated bonds. Of note, under RA treatment, TG 50:1, 52:2, 56:6and 60:8 became the most prominent.
Conclusion
Lipid metabolism is significantly different in the formation of cleft palate induced by RA, and the unsaturated triglycerides increased in the RA-treated group may play an important role in the formation of cleft palate.
期刊介绍：
Mechanisms of Development is an international journal covering the areas of cell biology and developmental biology. In addition to publishing work at the interphase of these two disciplines, we also publish work that is purely cell biology as well as classical developmental biology.
Mechanisms of Development will consider papers in any area of cell biology or developmental biology, in any model system like animals and plants, using a variety of approaches, such as cellular, biomechanical, molecular, quantitative, computational and theoretical biology.
Areas of particular interest include:
Cell and tissue morphogenesis
Cell adhesion and migration
Cell shape and polarity
Biomechanics
Theoretical modelling of cell and developmental biology
Quantitative biology
Stem cell biology
Cell differentiation
Cell proliferation and cell death
Evo-Devo
Membrane traffic
Metabolic regulation
Organ and organoid development
Regeneration
Mechanisms of Development does not publish descriptive studies of gene expression patterns and molecular screens; for submission of such studies see Gene Expression Patterns.
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