Larval Rearing Methods for Small-scale Production of Healthy Zebrafish.

Eastern biologist Pub Date : 2019-01-01
Alena Norton, Kitt F Franse, Tierney Daw, Latanya Gordon, Peter F Vitiello, Mary D Kinkel
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Abstract

Raising larvae of Danio rerio Hamilton (zebrafish) is a challenging task that requires skill and a significant daily time investment. We have developed a simple nursery and a husbandry regimen that streamlines procedures and is feasible for small laboratories to carry out in the absence of support staff. The nursery is inexpensive to build and easy to maintain. The regimen uses a simple benchtop nursery that houses up to 300 larvae. Feeding is simplified by using defined volumes of microencapsulated feeds with only 1 type of live prey as a dietary supplement, Artemia franciscana Leach (brine shrimp). Tests of the regimen using wild-type lines showed that it supports timely entry into the metamorphic period and supports survival rates of at least 75%. Further, inexperienced users were able to raise larvae successfully. Here, we describe how to assemble the nursery and how to carry out the feeding and care regimen.

Abstract Image

Abstract Image

Abstract Image

健康斑马鱼小规模生产的幼虫饲养方法。
饲养斑马鱼(Danio rerio Hamilton)的幼虫是一项具有挑战性的任务,需要技能和大量的日常时间投资。我们制定了一个简单的苗圃和饲养方案,简化了程序,在没有支助人员的情况下,小型实验室也可以进行。苗圃造价低廉,易于维护。该方法使用一个简单的台式苗圃,最多可容纳300只幼虫。通过使用限定体积的微胶囊饲料,仅将一种活猎物作为膳食补充,即Artemia franciscana Leach(盐水虾),从而简化了喂养。使用野生型系进行的试验表明,该方案支持及时进入变质期,并支持至少75%的存活率。此外,没有经验的使用者能够成功地饲养幼虫。在这里,我们描述如何组装苗圃和如何进行喂养和护理方案。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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