Development and evaluation of calcium hydroxide-coated, pericardium-based biomembranes for direct pulp capping

Q1 Medicine

Journal of Investigative and Clinical Dentistry Pub Date : 2018-12-07 DOI:10.1111/jicd.12380

Celso A. Klein-Júnior, Eduardo Reston, Ana M. Plepis, Virginia C. Martins, Isabel C. Pötter, Fionnuala Lundy, Guilherme S. Hentschke, Vítor S. Hentschke, Ikhlas El Karim

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引用次数: 11

Abstract

Aim

The aim of the present study was to develop a bovine pericardium biomembrane (BPB) and to evaluate pulp response in vivo.

Methods

A double-layer bovine BPB/chitosan was manufactured, and the porous chitosan side was coated with calcium hydroxide. The microstructure of the matrices was evaluated with electron microscopy. To test pulp response, cavities were prepared on the occlusal surface of Wistar rats’ mandibular left first molars and capped with matrices, followed by appropriate adhesives/composite restorations. The animals were divided into three groups: group 1, calcium hydroxide alone; group 2, BPB without calcium hydroxide; and group 3, BPB coated with calcium hydroxide. Specimens were processed and histologically evaluated at 7, 14, and 30 days, postoperatively.

Results

Electron microscopy showed porous chitosan surface and a cohesive calcium hydroxide layer. Histological analysis showed that groups 1 and 3 had mild odontoblast layer disorganization, but normal pulp tissue appearance at 7, 14, and 30 days. At the same time points, group 2 showed a loss of general pulp tissue, pulp necrosis, and periapical abscess in some teeth.

Conclusion

Coated bovine pericardium-based biomembranes resulted in favorable outcomes in cases of pulp exposure after a 30-day observation period, and might protect against injuries caused by adhesive systems and composites.

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氢氧化钙包覆心包生物膜直接盖髓的开发与评价

目的制备牛心包生物膜，并在体内评价其对生物膜的反应。方法制备双层牛BPB/壳聚糖，壳聚糖多孔侧包被氢氧化钙。用电子显微镜观察了基体的微观结构。为了测试牙髓反应，我们在Wistar大鼠下颌骨左第一磨牙的咬合面制备空腔并覆盖基质，然后使用合适的粘接剂/复合修复体。实验动物分为3组:1组单独给予氢氧化钙;第2组，不含氢氧化钙的BPB;第3组，包覆氢氧化钙的BPB。分别于术后7、14、30天对标本进行处理和组织学评价。结果壳聚糖表面呈多孔状，壳聚糖表面有粘连的氢氧化钙层。组织学分析显示，组1和组3在7、14和30 d时牙髓组织外观正常，成牙髓层轻度解体。在同一时间点，2组牙髓组织普遍丢失，牙髓坏死，部分牙尖周脓肿。结论包被的牛心包生物膜在牙髓暴露的情况下，经过30天的观察，具有良好的效果，并且可以防止胶粘剂系统和复合材料造成的损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Investigative and Clinical Dentistry DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.90

自引率

0.00%

发文量

期刊介绍： Journal of Investigative and Clinical Dentistry (JICD) aims to publish quality, peer reviewed original research and topical reviews on all aspects of investigative and clinical dentistry and craniofacial research, including molecular studies related to oral health and disease. Although international in outlook, the Editor especially encourages papers from the Asia Pacific. The journal also aims to provide clinicians, scientists and students of dentistry with a knowledge transfer platform for rapid publication of reports through an international journal, which will be available free online until 2012. Its scope, therefore, is broad, inclusive and international, but with a particular focus on Asia Pacific. The Editor welcomes manuscripts in the following key thematic areas in oral and maxillofacial sciences: Community Dentistry and Oral Epidemiology, Conservative Dentistry, Dental Biomaterials, Dental Pedagogy, Endodontics and Traumatology, Implant Dentistry, Oral Biosciences, Oral and Maxillofacial Surgery, Oral Medicine, Oral Pathology and Oral Microbiology, Orthodontics, Oral Radiology, Oral Rehabilitation, Paedodontics, Periodontology and Periodontal Medicine.