UHPLC-MS Analyses of Plant Flavonoids

Q1 Agricultural and Biological Sciences

Current protocols in plant biology Pub Date : 2018-11-29 DOI:10.1002/cppb.20085

Zhentian Lei, Barbara W. Sumner, Anil Bhatia, Saurav J. Sarma, Lloyd W. Sumner

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引用次数: 23

Abstract

Flavonoids are a class of specialized metabolites found in many different plant species. They protect against UV radiation, scavenge reactive oxygen species, are involved in plant defense responses, and are associated with plant-microorganism interactions. They have also been reported to possess health-promoting effects including anti-inflammatory, antioxidant, anticancer activity, and antihypertensive effects. Flavonoids encompass >10,000 structures where the types and amounts depend on the plant species, developmental stage, organ, and growth conditions. The diversity of flavonoid structures represents a significant challenge in the analysis of plant flavonoids. Many analytical techniques have been developed to detect and quantify flavonoids, and the most productive of these techniques use liquid chromatography (LC) coupled to mass spectrometry (MS) to analyze flavonoids due to the excellent combination of selectivity and sensitivity of MS. In addition, mass spectral libraries have been constructed to further aid flavonoid identification. Here, the use of ultra-high pressure liquid chromatography coupled to mass spectrometry (UHPLC-MS) in plant flavonoid analyses, with an emphasis on sample extraction, flavonoid separation, and MS detection, is described. © 2018 by John Wiley & Sons, Inc.

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植物总黄酮的UHPLC-MS分析

黄酮类化合物是一类特殊的代谢物，存在于许多不同的植物物种中。它们抵御紫外线辐射，清除活性氧，参与植物防御反应，并与植物与微生物的相互作用有关。据报道，它们还具有促进健康的作用，包括抗炎、抗氧化、抗癌活性和抗高血压作用。黄酮类化合物包含10,000种结构，其类型和数量取决于植物的种类、发育阶段、器官和生长条件。黄酮类化合物结构的多样性是植物黄酮类化合物分析的一个重大挑战。由于质谱联用技术具有良好的选择性和灵敏度，因此，目前已开发出多种检测和定量黄酮类化合物的分析技术，其中以液相色谱联用质谱法(MS)分析黄酮类化合物的效率最高。此外，质谱库的建立也有助于黄酮类化合物的鉴定。本文介绍了超高压液相色谱-质谱联用(UHPLC-MS)在植物类黄酮分析中的应用，重点介绍了样品提取、类黄酮分离和质谱检测。©2018 by John Wiley &儿子,Inc。

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来源期刊

Current protocols in plant biology Agricultural and Biological Sciences-Plant Science

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. Current Protocols in Plant Biology provides reproducible step-by-step instructions for protocols relevant to plant research. Furthermore, Current Protocols content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols in Plant Biology to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Plant Biology is the comprehensive source for protocols in the multidisciplinary field of plant biology, providing an extensive range of protocols from basic to cutting edge. Coverage includes: Extraction and analysis of DNA, RNA, proteins Chromosome analysis Transcriptional analysis Protein expression Metabolites Plant enzymology Epigenetics Plant genetic transformation Mutagenesis Arabidopsis, Maize, Poplar, Rice, and Soybean, and more.