Prevalence of Panton-Valentine Leukocidin Gene among Community Acquired Staphylococcus aureus: A Real-Time PCR Study.

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Accounts of Chemical Research Pub Date : 2018-09-02 eCollection Date: 2018-01-01 DOI:10.1155/2018/4518541
Amit Karmakar, Debarati Jana, Kunal Dutta, Parimal Dua, Chandradipa Ghosh
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引用次数: 0

Abstract

Panton-Valentine leukocidin (luk-pv) is a cytotoxin that causes leukocyte destruction and tissue necrosis. The aim of this study was to determine the prevalence of the pv1, mecA, and nuc genes in Staphylococcus aureus isolates obtained from anterior nares and superficial infection sites of skin in a slum population of West Bengal, India. Expression level of pv1 gene was also analysed. Twenty-two S. aureus strains were isolated, and phenotype and genotype specific examinations for S. aureus isolates were carried out. Molecular identification was done by PCR using species-specific 16S rRNA primer pairs and finally 22 isolates were found to be positive as S. aureus. The antibiotic responsiveness of all these isolates and the minimum inhibitory concentration (MIC) of MRSA isolates were determined using the broth dilution method with vancomycin. Antibiogram analysis of isolated S. aureus strains with respect to different antimicrobial agents revealed antibiotic resistance ranging from 27 to 91%. The results of MIC for vancomycin showed 95% of strains to be VSSA and 5% to be VISA. 68% isolates were resistant to methicillin. All the isolates were subjected to detection of pv1, mecA, and nuc genes, and 9%, 68%, and 27% were found to harbour pvl, mecA, and nuc genes, respectively. All the MRSA strains produced high to moderate levels of biofilm. pvl gene expression was carried out in vitro by Real-Time PCR. The low ∆Ct value (0.493) was indicative of high expression of pvl in one S. aureus strain. Thus, detection of pvl gene in community acquired S. aureus indicates the emergence of pathogenic S. aureus in community setup in the studied region. The existing exploration is extremely imperative and informative for the high level multi-drug resistant S. aureus infections inclusive of MRSA.

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社区获得性金黄色葡萄球菌中潘顿-瓦伦丁白细胞介素基因的流行率:实时 PCR 研究。
潘通-瓦伦丁白细胞介素(luk-pv)是一种细胞毒素,可导致白细胞破坏和组织坏死。本研究旨在确定从印度西孟加拉邦贫民窟人群的前鼻孔和皮肤浅表感染部位分离到的金黄色葡萄球菌中 pv1、mecA 和 nuc 基因的流行情况。还分析了 pv1 基因的表达水平。共分离出 22 株金黄色葡萄球菌,并对分离出的金黄色葡萄球菌进行了表型和基因型特异性检查。使用物种特异性 16S rRNA 引物对 PCR 进行分子鉴定,最终发现 22 株分离物为阳性金黄色葡萄球菌。使用万古霉素肉汤稀释法测定了所有这些分离物的抗生素反应性和 MRSA 分离物的最低抑菌浓度(MIC)。分离出的金黄色葡萄球菌菌株对不同抗菌剂的抗菌谱分析显示,抗生素耐药性从 27% 到 91% 不等。万古霉素的 MIC 结果显示,95% 的菌株为 VSSA 菌株,5% 为 VISA 菌株。68%的分离菌株对甲氧西林耐药。对所有分离株进行了 pv1、mecA 和 nuc 基因检测,发现分别有 9%、68% 和 27% 的分离株携带 pvl、mecA 和 nuc 基因。所有 MRSA 菌株都会产生高到中等程度的生物膜。低 ∆Ct 值(0.493)表明一株金黄色葡萄球菌的 pvl 基因表达量较高。因此,在社区获得的金黄色葡萄球菌中检测到 pvl 基因表明,在研究区域的社区设置中出现了致病性金黄色葡萄球菌。对于包括 MRSA 在内的高水平多重耐药金黄色葡萄球菌感染,现有的探索是非常必要和有参考价值的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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