[Effects of the Saponins from Panax japonicus on Intestinal Epithelial Tight Junction Proteins in Aging Rats].

中药材 Pub Date : 2016-09-01

Hai-ying Wang, Yao-yan Dun, Ding Yuan, Min Liu, Yu-hui Guo, Chang-cheng Zhang

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引用次数: 0

Abstract

Objective: To investigate the moderating effects of total saponins of Panax japonicus on intestinal epithelial tight junction proteins in aging rats,and to explore the potential mechanism.

Methods: SD rats were divided into adult group( 6 months),old model group( 24 months),and different doses( 10,30 and 60 mg / kg) of total saponins of Panax japonicus treatment groups. Levels of tight junction proteins( Occludin and ZO-1),anti-oxidative pathway proteins( Nrf2、HO-1 and NQO-1),mitochondrial biogenesis related proteins( Sirt1 and PGC-1α) and p-AMPK in the ileum were determined by immunohistochemistry staining.

Results: Compared with adult group,the expressions of Occludin,ZO-1,Nrf2,HO-1,NQO-1,Sirt1 and PGC-1α of aging rats were obviously decreased( P < 0. 01),and p-AMPK was inhibited in the ileum of aging rats. Compared with aging model rats,total saponins of Panax japonicus increased the expressions of Occludin,ZO-1,Nrf2,HO-1,NQO-1,Sirt1 and PGC-1α( P < 0. 05 or P < 0. 01),and activated p-AMPK in the ileum of aging rats.

Conclusion: The decreased level of intestinal tight junction proteins in the ileum of aging rats may be related to oxidative stress. Total saponins of Panax japonicus can up-regulate the level of intestinal tight junction proteins to improve the intestinal mucosal barrier dysfunction in the ileum of aging rat via reducing the levels of oxidative stress.

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[参总皂苷对衰老大鼠肠上皮紧密连接蛋白的影响]。

目的:研究三七总皂苷对衰老大鼠肠上皮紧密连接蛋白的调节作用，并探讨其可能的机制。方法:将SD大鼠分为成体组(6月龄)、老模型组(24月龄)和不同剂量(10、30、60 mg / kg)给药组。免疫组化染色检测回肠紧密连接蛋白(Occludin和ZO-1)、抗氧化途径蛋白(Nrf2、HO-1和NQO-1)、线粒体生物发生相关蛋白(Sirt1和PGC-1α)和p-AMPK水平。结果:与成年组比较，老龄大鼠Occludin、ZO-1、Nrf2、HO-1、NQO-1、Sirt1、PGC-1α表达明显降低(P < 0.05)。p-AMPK在衰老大鼠回肠中被抑制。与衰老模型大鼠相比，参总皂苷提高了Occludin、ZO-1、Nrf2、HO-1、NQO-1、Sirt1、PGC-1α的表达(P < 0.05)。0.05或P < 0。01)，激活衰老大鼠回肠p-AMPK。结论:衰老大鼠回肠紧密连接蛋白水平下降可能与氧化应激有关。三七总皂苷可通过降低氧化应激水平，上调肠道紧密连接蛋白水平，改善衰老大鼠回肠黏膜屏障功能障碍。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

中药材

自引率

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发文量

11364

期刊介绍： "Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.