The Frequency of Antibiotic Resistance and ESBLs Among Clinically Acinetobacter baumannii Strains Isolated from Patients in a Major Hospital in Tehran, Iran.

Q3 Immunology and Microbiology

Open Microbiology Journal Pub Date : 2018-07-31 eCollection Date: 2018-01-01 DOI:10.2174/1874285801812010254

Reza Ranjbar, Sajjad S Tolon, Shahin Zayeri, Mehrdad Sami

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引用次数: 11

Abstract

Background: Bacterial resistance to antibiotics limits treatment options, increases morbidity and mortality, and raises the risk of antibiotic-associated adverse events. Antibacterial resistance emerges rapidly following an increase in the consumption of antibiotics against infectious diseases. The spread of ESBL producing strains has a limiting factor based on antibiotic function for the treatment of infections particularly caused by Acinetobacter baumannii (A. baumannii).

Objective: This study was conducted to evaluate the prevalence of antimicrobial resistance and distribution of bla_TEM , bla_CTX , and bla_SHV genes among A. baumannii strains isolated from clinical samples at a major hospital in Teheran, Iran.

Methods: A. baumannii strains were isolated and identified using standard microbiological methods. The disc diffusion and combined discs methods were used for testing antimicrobial susceptibility and to identify the strains producing Extended-Spectrum Beta-Lactamases (ESBL), respectively. DNA extraction was done by boiling method. Finally, the frequency of resistant genes including bla_TEM , bla_CTX , and bla_SHV in ESBL producing isolates was studied by PCR.

Results: Gender distribution in this study was 53 (53%) samples for men and 47 (47%) for women. Totally, one hundred A. baumannii strains were isolated. More than 93% of the isolates were multi drug resistant. The highest to lowest antibiotic resistance was observed against amoxicillin/clavulanic acid (98%), ceftriaxone (96%), cefotaxime (94%), and ceftazidime (93%), respectively. The frequency of positive phenotypic test of ESBL was 19% and 16% for CAZ-C and CTX-C, respectively. The frequency of bla_TEM , bla_CTX , and bla_SHV genes was 52.1, 43.4, and 21.7, respectively.

Conclusion: A. baumannii isolates exhibited an extremely worrying level of antibiotic resistance, and a high percentage of the isolates showed MDR in this study. This is a serious warning because ESBLs are a major threat to the effectiveness of antibiotics that are currently available for medical uses. The frequency of genes encoded ESBL isolates of A. baumannii may be due to overuse and misuse of antibiotics.

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伊朗德黑兰某大医院临床鲍曼不动杆菌耐药频率及ESBLs分析

背景:细菌对抗生素的耐药性限制了治疗选择，增加了发病率和死亡率，并增加了抗生素相关不良事件的风险。随着抗传染病抗生素消费量的增加，抗菌药物耐药性迅速出现。基于抗生素治疗感染的功能，产生ESBL的菌株的传播具有限制因素，特别是鲍曼不动杆菌(鲍曼不动杆菌)引起的感染。目的:研究伊朗德黑兰某大医院鲍曼不动杆菌临床分离株中blaCTX、blaSHV和blaCTX基因的耐药情况及分布。方法:采用标准微生物学方法对鲍曼不动杆菌进行分离鉴定。采用纸片扩散法和联合纸片法分别进行药敏试验和产广谱β -内酰胺酶(ESBL)菌株鉴定。采用煮沸法提取DNA。最后，采用PCR方法对ESBL产株中blaTEM、blaCTX和blaSHV耐药基因的频率进行分析。结果:本研究性别分布为男性53例(53%)，女性47例(47%)。共分离到鲍曼不动杆菌100株。93%以上的分离株具有多重耐药。耐药率最高至最低的分别是阿莫西林/克拉维酸(98%)、头孢曲松(96%)、头孢噻肟(94%)和头孢他啶(93%)。CAZ-C和CTX-C的ESBL表型检测阳性率分别为19%和16%。blaTEM、blaCTX和blaSHV基因的频率分别为52.1、43.4和21.7。结论:鲍曼不动杆菌分离株表现出非常令人担忧的抗生素耐药性水平，并且在本研究中，很高比例的分离株表现出耐多药。这是一个严重的警告，因为esbl是对目前可用于医疗用途的抗生素有效性的主要威胁。鲍曼不动杆菌ESBL分离株编码基因的频率可能是由于抗生素的过度使用和误用。

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来源期刊

Open Microbiology Journal Immunology and Microbiology-Immunology and Microbiology (all)

CiteScore

1.80

自引率

0.00%

发文量

期刊介绍： The Open Microbiology Journal is a peer-reviewed open access journal which publishes research articles, reviews/mini-reviews, case studies, guest edited thematic issues and short communications/letters covering theoretical and practical aspects of Microbial systematics, evolutionary microbiology, immunology, virology, parasitology , bacteriology, mycology, phycology, protozoology, microbial ecology, molecular biology, microbial physiology, biochemistry, microbial pathogenesis, host-microbe interaction, systems microbiology, synthetic microbiology, bioinformatics. The Open Microbiology Journal , a peer-reviewed journal, is an important and reliable source of current information on developments in the field. The emphasis will be on publishing quality papers rapidly and freely available to researchers worldwide.