[Study on Subcritical Fluid Extraction of Essential Oil from Cinnamomum camphora and Its Antibacterial Activity].

中药材 Pub Date : 2016-06-01

Hai-xu Zhou, Zhong-hai Li, Xiang-jin Fu, Hui Zhang

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引用次数: 0

Abstract

Objective: To determine the optimum extract condition of essential oil from dry Cinnamomum camphora leaves and to study its antibacterial activity.

Methods: The essential oil was extracted by subcritical fluid, and analyzed by response surface methodology based on single factor test. The chemical compositions of essential oil were analyzed by GC-MS, and the components were quantitatively determined by normalization method. The agar disc diffusion and dilution broth method were used to determine antibacterial activity.

Results: The extraction rate was highest as 3. 54%,which matched with the predictive yield of 3. 56%,and the extraction time was30 min, the extraction temperature was 40 ℃,and resolution temperature was 65 ℃. 47 chemical constituents were identified and isolated from Cinnamomum camphora, which was analyzed by GC-MS. The main chemical constituents were eucalyptol( 24. 74%),bicyclo[3. 1. 0]hexan-4-methylene-1-( 1-methylethyl) ( 7. 05%),linalool( 5. 82%),caryophyllene ( 4. 75%). The essential oil from Cinnamomum camphora had different degrees of inhibition on Escherichia coli,Staphylococcus aureus, Pseudomonas aeruginosa,Bacillus subtilis.

Conclusion: Subcritical fluid is suitable for the extraction of essential oil from Cinnamomum camphora and the essential oil has a good antimicrobial activity.

本刊更多论文

[亚临界流体萃取香樟精油及其抑菌活性的研究]。

目的:确定干香樟叶精油的最佳提取工艺，并研究其抑菌活性。方法:采用亚临界流体萃取法提取挥发油，采用单因素试验的响应面法进行分析。采用气相色谱-质谱法分析挥发油的化学成分，采用归一化法定量测定挥发油的成分。采用琼脂盘扩散法和稀释肉汤法测定抑菌活性。结果:提取率最高，为3。54%，与预测收益率3。提取时间为30 min，提取温度为40℃，分离温度为65℃。从樟树中分离鉴定了47种化学成分，并采用气相色谱-质谱法对其进行了分析。主要化学成分为桉树醇(24;74%), bicyclo[3。1. [7]己烷-4-亚甲基-1-(1-甲基乙基)05%)、芳樟醇(5。82%)，石竹烯(4%)。75%)。樟树精油对大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌、枯草芽孢杆菌均有不同程度的抑制作用。结论:亚临界液适用于香樟精油的提取，且香樟精油具有良好的抑菌活性。

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来源期刊

中药材

自引率

0.00%

发文量

11364

期刊介绍： "Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.