[Effects of Wogonin on Apoptosis,Invasion,Migration and Wnt/β-Catenin Signaling Pathway of Gastric Cancer Cells SGC7901].

中药材 Pub Date : 2016-06-01

Xiao-hui Wang, Ying-fang Wei, Hong Cheng

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引用次数: 0

Abstract

Objective: To explore the effects of wogonin on the apoptosis,invasion,migration and Wnt / β-catenin signaling pathway of gastric cancer cells SGC7901.

Methods: Three common gastric cancer cell lines( SGC7901,BGC-823 and MKN-45) were conventionally cultured to logarithmic growth. The cell proliferation was detected by MTT assay after treatment with different concentrations of wogonin( 0,20,50,100 and 200 μmol / L) for 24,48,72 and 96 h. The apoptosis rate, migration and invasion ability of SGC7901 cells were detected by Annexin V-FITC / PI annexin double staining flow cytometry, scratch test and transwell cell invasion assay, respectively. After treament with different concentrations of wogonin for 48 h,the protein levels of β-catenin, C-myc and Cyclin D1 were detected by Western blotting.

Results: Wogonin within the range of 20 ~ 200 mol / L could inhibit the proliferation of SGC7901,BGC-823 and MKN-45 cells in a dose-and time-dependent manner. Compared with 0 μmol / L wogonin group,After treament with different concentrations of wogonin for 24 h and 48 h,SGC7901 cells had elevated apoptosis rate and decreased migration distance and number of penetrating membrane cell in the rest concentrations( P < 0. 05). The protein levels of β-catenin,C-myc and Cyclin D1 were lower in 20 ~ 200μmol /L wogonin groups than 0 μmol /L wogonin group except for the concentration of 20 μmol /L wogonin groups( P < 0. 05),and the effects in a dose-dependent manner.

Conclusion: Wogonin has the ability to inhibit the proliferation of gastric cancer cells,and can induce apoptosis and inhibit cell migration and invasion,which may be related to the inhibition of Wnt / β-catenin signaling pathway activation.

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[Wogonin对胃癌细胞SGC7901凋亡、侵袭、迁移及Wnt/β-Catenin信号通路的影响]。

目的:探讨枸杞子素对胃癌细胞SGC7901凋亡、侵袭、迁移及Wnt / β-catenin信号通路的影响。方法:对3株常见胃癌细胞株SGC7901、BGC-823和MKN-45进行对数培养。采用MTT法检测不同浓度wogonin(0、20、50、100和200 μmol / L)处理SGC7901细胞24、48、72和96 h后的细胞增殖情况，采用Annexin V-FITC / PI双染色流式细胞术、划痕实验和transwell细胞侵袭实验检测SGC7901细胞的凋亡率、迁移和侵袭能力。不同浓度wogonin作用48 h后，采用Western blotting检测β-catenin、C-myc和Cyclin D1蛋白水平。结果:Wogonin在20 ~ 200 mol / L范围内可抑制SGC7901、BGC-823和MKN-45细胞的增殖，且呈剂量和时间依赖性。与0 μmol / L沃果苷组比较，不同浓度沃果苷处理24h和48h后，其余浓度下SGC7901细胞凋亡率升高，迁移距离和穿膜细胞数量减少(P < 0.05)。05）. 除20 μmol /L枸杞素组外，20 ~ 200μmol /L枸杞素组β-catenin、C-myc和Cyclin D1蛋白水平均低于0μmol /L枸杞素组(P < 0.05)。05)，且效果呈剂量依赖性。结论:Wogonin具有抑制胃癌细胞增殖、诱导细胞凋亡、抑制细胞迁移和侵袭的作用，可能与抑制Wnt / β-catenin信号通路激活有关。

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来源期刊

中药材

自引率

0.00%

发文量

11364

期刊介绍： "Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.