[Reactive oxygen and nitrogen species’ effect on lux-biosensors based on Escherichia coli and Salmonella typhimurium].

D N Karimova, I V Manukhov, E Yu Gnuchikh, I F Farimov, D G Deryabin
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Abstract

The effect of reactive oxygen and nitrogen species on lux-biosensors based on the Escherichia coli K12 MG1655 and Salmonella typhimurium LT2 host strains was investigated. The bioactivity of exogenous free radicals to the constitutively luminescent E. coli strain with plasmid pXen7 decreased in the order H2O2 > OCl– > NO• > RОO• > ONOO–> O 2 •- while the bioluminescence of S. typhimurium strain transformed with this plasmid decreased in the order NO• > H2O2 > ONOO– > RОO• > OCl– > O 2 •- The cross-reactivity of induced lux-biosensors to reactive oxygen and nitrogen species, the threshold sensitivity and the luminescence amplitude dependences from the plasmid specificity and the host strain were indicated. The biosensors with plasmid pSoxS′::lux possessed a wider range of sensitivity, including H2O2 and OCl–, along with O 2 •- and NO•. Among the used reactive oxygen and nitrogen species, H2O2 showed the highest induction activity concerning to the plasmids pKatG′::lux, pSoxS′::lux and pRecA′::lux. The inducible lux-biosensors based on S. typhimurium host strain possessed a higher sensitivity to the reactive oxygen and nitrogen species in comparison than the biosensors based on E. coli. .

[活性氧和氮对基于大肠杆菌和鼠伤寒沙门菌的奢华生物传感器的影响]。
以大肠杆菌K12 MG1655和鼠伤寒沙门菌LT2为宿主菌株,研究了活性氧和活性氮对奢侈生物传感器的影响。外源自由基对含有pXen7质粒的组成发光大肠杆菌菌株的生物活性降低的顺序为H2O2 > OCl - > NO•> RОO•> ONOO - > O2•-,而用该质粒转化的鼠伤寒沙门氏菌菌株的生物发光性降低的顺序为NO•> H2O2 > ONOO - > RОO•> OCl - > O2•-。指出了阈值灵敏度和发光幅度依赖于质粒特异性和宿主菌株。质粒pSoxS’::lux对H2O2、OCl -、o2•-、NO•均具有较高的灵敏度。在使用的活性氧和活性氮中,H2O2对pKatG '::lux、pSoxS '::lux和pRecA '::lux的诱导活性最高。与大肠杆菌相比,以鼠伤寒沙门氏菌为宿主菌株的诱导型lux-生物传感器对活性氧和活性氮具有更高的敏感性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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