DNA Microarray Analysis of Estrogen Responsive Genes in Ishikawa Cells by Glabridin.

Biochemistry Insights Pub Date : 2017-07-26 eCollection Date: 2017-01-01 DOI:10.1177/1178626417721676

Poh Su Wei Melissa, Yong Voon Chen Phelim, Visweswaran Navaratnam, Chia Yoke Yin

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引用次数: 4

Abstract

Based on a previous study, glabridin displayed a dose-dependent increase in estrogenic activity and cell proliferative activity in Ishikawa cells. However, when treated in combination with 17β-E₂, synergistic estrogenic effect was observed but without the same synergistic increase in cell proliferative effect. This study aimed to identify the estrogen and nonestrogen-regulated activities induced by glabridin and in combination with 17β-E₂ in comparison with 17β-E₂. The results showed that 10 µM glabridin and the combination treatment of 100 nM glabridin with 1 nM 17β-E₂ regulated both the genomic and nongenomic estrogen pathways to possibly provide benefits of estrogens in cardiovascular, circulatory, and vasculature systems. Meanwhile, the combination of 100 nM glabridin with 1 nM 17β-E₂ seems to be more suitable to be used as an estrogen replacement. Finally, the results of this study have added on to the present knowledge of glabridin's function as a phytoestrogen and suggested new ideas for the usage of glabridin.

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光甘草定对石川细胞雌激素应答基因的DNA芯片分析。

根据先前的一项研究，光甘草定在石川细胞中显示出剂量依赖性的雌激素活性和细胞增殖活性增加。然而，当与17β-E2联合治疗时，观察到增效雌激素作用，但没有相同的增效细胞增殖作用。本研究旨在鉴定光甘草定及其与17β-E2联合使用对雌激素和非雌激素调节活性的影响，并与17β-E2进行比较。结果表明，10µM光甘草定和100 nM光甘草定与1 nM 17β-E2联合治疗可调节基因组和非基因组雌激素通路，可能在心血管、循环和血管系统中提供雌激素的益处。同时，100 nM光定与1 nM 17β-E2联合使用似乎更适合作为雌激素替代品。最后，本研究的结果增加了光甘草定作为植物雌激素功能的现有知识，并为光甘草定的使用提出了新的思路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biochemistry Insights BIOCHEMISTRY & MOLECULAR BIOLOGY-

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