Alcoholic Steatosis in Different Strains of Rat: A Comparative Study.

Q4 Psychology

Journal of Drug and Alcohol Research Pub Date : 2015-01-01 Epub Date: 2015-05-25 DOI:10.4303/jdar/235912

Kamlesh K Bhopale, Shakuntala Kondraganti, Harshica Fernando, Paul J Boor, Bhupendra S Kaphalia, G A Shakeel Ansari

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引用次数: 4

Abstract

Background: Different strains of rats have been used to study alcoholic liver disease (ALD) while the reason for selecting a particular rat strain was not apparent.

Purpose: The aim of our study was to compare outbred (Wistar) and inbred (Fischer) strains to evaluate pathological, biochemical changes, and gene expression differences associated with ethanol-induced early hepatic steatosis.

Study design: Male Wistar and Fischer-344 rats were pair-fed for 6 weeks with or without 5% ethanol in Lieber-DeCarli liquid diet. Livers were analyzed for histological and lipid-related differences.

Results: Hepatic midzonal steatosis was mainly found in Wistar rats while Fischer rats showed mostly pericentral steatosis. Increased hepatic steatosis in ethanol-fed Wistar rats is supported by increases in lipids with related genes and transcription factors involved in fatty acid and triglyceride synthesis.

Conclusion: Our data showed that Fischer rats are relatively less prone to ethanol-mediated steatosis with pericentral lipid deposition pattern in the liver which is similar to humans and show no trace level of lipid accumulation in pair-fed controls as observed in Wistar (outbred) strain. Therefore, Fischer rats are better suited for lipid studies in an early development of ALD.

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不同品系大鼠酒精性脂肪变性的比较研究

背景:不同品系的大鼠已被用于酒精性肝病(ALD)的研究，而选择特定品系的原因尚不清楚。目的:本研究的目的是比较近交系(Wistar)和近交系(Fischer)菌株，以评估乙醇诱导的早期肝脂肪变性的病理、生化变化和基因表达差异。研究设计:雄性Wistar大鼠和fisher -344大鼠在Lieber-DeCarli液体饲料中添加或不添加5%乙醇，成对饲养6周。分析肝脏的组织学和脂质相关差异。结果:Wistar大鼠以肝中区脂肪变性为主，Fischer大鼠以中心周围脂肪变性为主。与脂肪酸和甘油三酯合成相关基因和转录因子相关的脂质增加支持了乙醇喂养Wistar大鼠肝脂肪变性的增加。结论:我们的数据显示，Fischer大鼠相对不容易发生乙醇介导的脂肪变性，其肝脏中心周围脂肪沉积模式与人类相似，并且在Wistar(近交种)品系中观察到的配对喂养对照组中没有微量的脂肪积累。因此，Fischer大鼠更适合于ALD早期发展的脂质研究。

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来源期刊

Journal of Drug and Alcohol Research Psychology-Clinical Psychology

CiteScore

0.50

自引率

0.00%

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期刊介绍： The Journal of Drug and Alcohol Research (JDAR) is a scholarly open access, peer-reviewed, and fully refereed journal dedicated to publishing sound papers on advances in the field of drug, opiate, nicotine and alcohol abuse, both basic and clinical. The journal will consider papers from all sub-disciplines and aspects of drug abuse, dependence and addiction research. Manuscripts will be published online as soon as they are accepted, which will reduce the time of publication. Because there are no space limitations or favored topics, all papers, within the scope of the journal, judged to be sound by the reviewers, will be published.