Histopathology and Histomorphometric Investigation of Bisphenol A and Nonylphenol on the Male Rat Reproductive System.

Abstract

Background: Bisphenol A (BPA) and nonylphenol (NP) have harmful effects on the endocrine system of humans and animals.

Aim: We sought to investigate the effect of three doses of BPA and NP on the reproductive parameters of rats.

Materials and methods: Adult Wistar male rats weighing 150-200 g were used for a consecutive 35-day study. BPA and NP were given as gavage in three doses (5, 25, and 125 μg/kg). At the end of the study, the rats were anesthetized and 2 ml blood sample was obtained from the auxiliary venous plexus for the assessment of sex hormone levels. The testes were removed and kept in 10% formalin for the histomorphometric and histopathologic analyses.

Results: BPA and NP significantly decreased the body weight of the animals compared to the controls (P < 0.05). The seminiferous tubule diameter and thickness of the seminiferous epithelium were significantly decreased in the groups receiving BPA and NP compared to the control (P < 0.05). The number of seminiferous tubules in every experimental group increased, except for the highest dose of NP (125 μg/kg), which showed a significant difference compared to the controls (P < 0.05). The number of spermatocytes and spermatogonia (54.97 ± 5.824, 35.78 ± 3.956, respectively) in the group receiving NP (125 μg/kg) was significantly decreased compared to the other groups. Serum levels of luteinizing hormone, Follicle stimulating hormone, and testosterone did not show any significant change compared to the controls.

Conclusion: Based on the results, all three doses of BPA and NP significantly produced weight loss, as well as destruction of the testis tissue and impairment of the spermatogenesis.