Diffuse large B-cell lymphoma: morphologic and immunohistochemical analysis of bone marrow for staging, with emphasis on lymphoid aggregates.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-09-15 eCollection Date: 2022-01-01

Vinicius C Nóbrega, Maria A C Domingues, Lígia Niero-Melo, Cristiano C Oliveira

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引用次数: 0

Abstract

Diffuse large B-Cell lymphoma (DLBCL) may infiltrate bone marrow (BM) and evaluation of BM plays an important role in DLBCL staging. This study used BM samples from DLBCL patients for staging and analyzed the use of immunohistochemistry in the diagnostic management of these cases by the pathologist. Patients with DLBCL submitted to BM biopsy/aspiration for staging were studied according to clinical aspects, morphologic aspects, and expression of CD20 and CD3. The characteristics of lymphoid aggregates in the bone marrow and the power of histopathological diagnosis were studied, with immunohistochemistry as the gold standard for the decision of a neoplastic infiltration definition. An isolated morphological analysis showed low sensitivity (42.9%) for lymphoma detection in BM, which is disadvantageous. The median of three lymphoid aggregates in the BM (p-value = 0.02) and the presence of increased reticulin fibers (grade 2) in the lymphoid aggregate (p-value = 0.01) had significant associations with neoplastic infiltration. A morphological analysis must be accompanied by an immunohistochemical analysis in all cases, or when this is not possible, in cases with two or more lymphoid aggregates or an increase of reticulin within them.

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弥漫性大b细胞淋巴瘤:骨髓形态学和免疫组织化学分析的分期，重点是淋巴样聚集体。

弥漫性大b细胞淋巴瘤(DLBCL)可浸润骨髓(BM)，骨髓的评估在DLBCL分期中起重要作用。本研究使用来自DLBCL患者的BM样本进行分期，并分析了病理学家在这些病例的诊断管理中使用免疫组织化学。根据临床、形态学、CD20和CD3的表达对经BM活检/抽吸的DLBCL患者进行分期研究。研究了骨髓淋巴细胞聚集的特征和组织病理学诊断的力量，以免疫组织化学作为决定肿瘤浸润定义的金标准。孤立的形态学分析显示，BM中淋巴瘤的检测灵敏度较低(42.9%)，这是不利的。BM中三个淋巴聚集体的中位数(p值= 0.02)和淋巴聚集体中网状蛋白纤维(2级)的增加(p值= 0.01)与肿瘤浸润有显著关联。形态学分析必须伴随免疫组织化学分析在所有情况下，或当这是不可能的，在两个或更多淋巴样聚集体或其中网状蛋白增加的情况下。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.