Bufei Decoction Improves Lung-Qi Deficiency Syndrome of Chronic Obstructive Pulmonary Disease in Rats by Regulating the Balance of Th17/Treg Cells.

4区医学 Q2 Medicine

Evidence-based Complementary and Alternative Medicine Pub Date : 2022-08-17 eCollection Date: 2022-01-01 DOI:10.1155/2022/1459232

Junxi Shen, Xing Zhu, Yunzhi Chen, Wen Li, Huaiquan Liu, Cancan Chu, Yu Zhang, Changjun Xu, Pingzhen Tong, Xinran Yu, Guangyong Yang, Ying Deng

{"title":"Bufei Decoction Improves Lung-Qi Deficiency Syndrome of Chronic Obstructive Pulmonary Disease in Rats by Regulating the Balance of Th17/Treg Cells.","authors":"Junxi Shen, Xing Zhu, Yunzhi Chen, Wen Li, Huaiquan Liu, Cancan Chu, Yu Zhang, Changjun Xu, Pingzhen Tong, Xinran Yu, Guangyong Yang, Ying Deng","doi":"10.1155/2022/1459232","DOIUrl":null,"url":null,"abstract":"Bufei decoction (BFD) has been applied to treat chronic obstructive pulmonary disease (COPD) for centuries as a recognized traditional Chinese herbal formula. However, mechanisms of BFD on COPD are unclear. This study conducts an inquiry into the underlying mechanisms of the therapeutic effect of BFD on COPD. A COPD rat model with qi deficiency in lungs was established through induction using cigarette and sawdust smoking combined with intratracheal instillation of lipopolysaccharide following BFD treatment for 28 days. Changes in Th17/Treg cells of COPD rats with the syndrome of lung qi deficiency after BFD administration were verified using pulmonary function, ELISA, flow cytometry, histopathology, and Western blotting assays. The findings showed that BFD protected COPD rats from decreased lung function and lung injury. BFD administration reduced proinflammatory cytokines IL-6 and IL-17 secretion, promoted inhibitory cytokines IL-10 and TGF-β secretion, decreased Th17/Treg cell ratio, markedly downregulated the Th17 cell transcription factor ROR-γt expression, and upregulated transcription factor Foxp3 expression in Treg cells. We speculate that lung tonic soup improved pulmonary qi deficiency in rats with COPD by regulating the balance of Th17/Treg cells.","PeriodicalId":12236,"journal":{"name":"Evidence-based Complementary and Alternative Medicine","volume":" ","pages":"1459232"},"PeriodicalIF":0.0000,"publicationDate":"2022-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9402293/pdf/","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Evidence-based Complementary and Alternative Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1155/2022/1459232","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 1

Abstract

Bufei decoction (BFD) has been applied to treat chronic obstructive pulmonary disease (COPD) for centuries as a recognized traditional Chinese herbal formula. However, mechanisms of BFD on COPD are unclear. This study conducts an inquiry into the underlying mechanisms of the therapeutic effect of BFD on COPD. A COPD rat model with qi deficiency in lungs was established through induction using cigarette and sawdust smoking combined with intratracheal instillation of lipopolysaccharide following BFD treatment for 28 days. Changes in Th17/Treg cells of COPD rats with the syndrome of lung qi deficiency after BFD administration were verified using pulmonary function, ELISA, flow cytometry, histopathology, and Western blotting assays. The findings showed that BFD protected COPD rats from decreased lung function and lung injury. BFD administration reduced proinflammatory cytokines IL-6 and IL-17 secretion, promoted inhibitory cytokines IL-10 and TGF-β secretion, decreased Th17/Treg cell ratio, markedly downregulated the Th17 cell transcription factor ROR-γt expression, and upregulated transcription factor Foxp3 expression in Treg cells. We speculate that lung tonic soup improved pulmonary qi deficiency in rats with COPD by regulating the balance of Th17/Treg cells.

Abstract Image

查看原文本刊更多论文

不老煎通过调节Th17/Treg细胞的平衡改善慢性阻塞性肺病大鼠的肺气虚综合征

数百年来，布非煎（BFD）作为一种公认的传统中药配方，一直被用于治疗慢性阻塞性肺病（COPD）。然而，布斐对慢性阻塞性肺病的作用机制尚不清楚。本研究探讨了 BFD 对慢性阻塞性肺病治疗作用的内在机制。通过使用香烟和锯末吸烟诱导慢性阻塞性肺病大鼠，并在 BFD 治疗 28 天后气管内灌注脂多糖，建立了肺部气虚的慢性阻塞性肺病大鼠模型。通过肺功能、ELISA、流式细胞术、组织病理学和Western印迹检测，验证了BFD治疗后COPD大鼠肺气虚综合征Th17/Treg细胞的变化。研究结果表明，BFD 能保护慢性阻塞性肺病大鼠免受肺功能下降和肺损伤的影响。BFD能减少促炎细胞因子IL-6和IL-17的分泌，促进抑制细胞因子IL-10和TGF-β的分泌，降低Th17/Treg细胞的比例，明显下调Th17细胞转录因子ROR-γt的表达，上调Treg细胞转录因子Foxp3的表达。我们推测补肺汤是通过调节Th17/Treg细胞的平衡来改善慢性阻塞性肺病大鼠的肺气不足。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Evidence-based Complementary and Alternative Medicine 医学-全科医学与补充医学

自引率

0.00%

发文量

1983

审稿时长

2.2 months

期刊介绍： Evidence-Based Complementary and Alternative Medicine (eCAM) is an international, peer-reviewed journal that seeks to understand the sources and to encourage rigorous research in this new, yet ancient world of complementary and alternative medicine. The journal seeks to apply scientific rigor to the study of complementary and alternative medicine (CAM) modalities, particularly traditional Asian healing systems. eCAM emphasizes health outcome, while documenting biological mechanisms of action. The journal is devoted to the advancement of science in the field of basic research, clinical studies, methodology or scientific theory in diverse areas of Biomedical Sciences. The journal does not consider articles on homeopathy.