Protective effect of ghrelin in a rat model of celiac disease.

L R Nikoukar, Fatemeh Nabavizadeh, S M Mohamadi, A Moslehi, G Hassanzadeh, H Nahrevanian, S Agah
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引用次数: 14

Abstract

Ghrelin is a gut hormone shown to have protective effects throughout the gastrointestinal tract. This study aims to investigate its protective effect in celiac disease induced in rats. Twenty-four rat pups were divided into 4 groups as follows: control, disease (1.5 mg/g intragastric gliadin), co-treatment (50 ng/g intraperitoneal ghrelin after gliadin gavage) and pretreatment (50 ng/g intraperitoneal ghrelin before gliadin gavage). Animals' weight gain was charted. Histological features assessed include villus length, villus width, crypt depth and number of intraepithelial lymphocytes. Tissue interferon-gamma was quantified by ELISA. ANOVA was used to compare results statistically. Results showed that villi were shortened in the diseased group, but were as long as the control in pretreatment and co-treatment groups. Crypt depth had increased in disease group, but turned to normal in co-treatment group. Number of intraepithelial lymphocytes was significantly higher in disease group than the control, while no difference was observed between co-treatment and control groups. Disease and control animals weighed equally at the end of the experiment, but ghrelin-treated animals had significantly gained more weight than these two. Interferon-gamma measurement revealed no significant difference among groups. We concluded administration of ghrelin led to histological improvement of celiac disease which was more obvious if administered after exposure to gliadin.

胃饥饿素对腹腔疾病大鼠模型的保护作用。
胃饥饿素是一种肠道激素,对整个胃肠道都有保护作用。本研究旨在探讨其对大鼠腹腔疾病的保护作用。将24只大鼠幼崽分为4组,分别为对照组、疾病组(麦胶蛋白1.5 mg/g灌胃)、共处理组(麦胶蛋白灌胃后腹腔注射50 ng/g胃饥饿素)和预处理组(麦胶蛋白灌胃前腹腔注射50 ng/g胃饥饿素)。动物的体重增加被绘制成图表。组织学特征包括绒毛长度、绒毛宽度、隐窝深度和上皮内淋巴细胞数量。ELISA法定量组织γ干扰素。采用方差分析对结果进行统计学比较。结果显示,病变组绒毛缩短,而预处理组和联合治疗组绒毛长度与对照组相当。病变组隐窝深度增加,联合治疗组隐窝深度恢复正常。疾病组上皮内淋巴细胞数量明显高于对照组,而联合治疗组与对照组无差异。实验结束时,疾病组和对照组的体重相等,但使用生长素的动物的体重明显高于这两组。干扰素测量结果显示各组间无显著差异。我们得出结论,胃饥饿素的使用导致乳糜泻的组织学改善,如果在接触麦胶蛋白后使用,这种改善更为明显。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Acta physiologica Hungarica
Acta physiologica Hungarica 医学-生理学
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6.0 months
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