{"title":"Sex chromatin staining in amnion cells.","authors":"Blanche Capel, Jordan Batchvarov","doi":"10.1101/pdb.prot5079","DOIUrl":null,"url":null,"abstract":"<p><p>INTRODUCTIONThe procedure described here provides a quick and reliable method for determining the sex of mouse embryos that are <12.5 days post-coitum (dpc). Cells from amniotic membranes are stained with toluidine blue. The presence of a heavily stained condensed chromatin body (i.e., a Barr body) indicates the XX samples. With experience, we find a >95% concordance with genotyping data based on PCR for Y-chromosome sequences in extracted tail DNA. This protocol has the advantage of speed and efficiency: When assembling cultures with live tissue, samples can be sexed in 30 min.</p>","PeriodicalId":10835,"journal":{"name":"CSH protocols","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2008-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1101/pdb.prot5079","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"CSH protocols","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1101/pdb.prot5079","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
INTRODUCTIONThe procedure described here provides a quick and reliable method for determining the sex of mouse embryos that are <12.5 days post-coitum (dpc). Cells from amniotic membranes are stained with toluidine blue. The presence of a heavily stained condensed chromatin body (i.e., a Barr body) indicates the XX samples. With experience, we find a >95% concordance with genotyping data based on PCR for Y-chromosome sequences in extracted tail DNA. This protocol has the advantage of speed and efficiency: When assembling cultures with live tissue, samples can be sexed in 30 min.
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