[Application of a simple method for detection of rubella virus genome by loop-mediated isothermal amplification (LAMP)].

Abstract

Objective: To use a new simple Reverse Transcription Loop-mediated isothermal amplification (RT-LAMP) method was applied to detect rubella virus nucleic acid and compared with Reverse Transcription-Polymerase Chain Reaction (RT-PCR).

Method: Comparing the detection rate of the RT-LAMP method with that of RT-PCR for detecting rubella virus nucleic acid from rubella virus.

Results: The nucleic acid positive rates of all 11 strains of rubella virus were 100% by the two methods, the positive rate was 55%.

Conclusion: RT-LAMP is more simple and convenient than RT-PCR.