Determination of flavonoids in Plumula Nelumbini by micellar electrokinetic capillary electrophoresis with electrochemical detection.

Abstract

A simple and efficient method based on micellar electrokinetic capillary electrophoresis (MECC) with electrochemical detection for the determination of catechin, rutin, hyperin, and kaempferol in Plumula Nelumbini is described. Under optimum conditions, all analytes were well separated within 20 min at a separation voltage of 16 kV in a 60-mmol/L borate running buffer (pH 8.0) containing 20 mmol/L sodium dodecyl sulfate (SDS). The current response was linear over two orders of magnitude with detection limits (S/N = 3) ranging from 6.90 x 10(-8) g/mL to 1.30 x 10(-7) g/mL for all analytes. This work provides a useful method for the analysis of Plumula Nelumbini.