Inhibitory effects of selenium on telomerase activity and hTERT expression in cadmium-transformed 16HBE cells.

Biomedical and environmental sciences : BES Pub Date : 2007-08-01

Hua-Jie Chen, Ri-An Yu, Ling-Fei He, She-Juan An, Zhi-Gang Wu, Ke-Di Yang, Xue-Min Chen

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Abstract

Objective: To investigate the effects of sodium selenite on telomerase activity and expression of hTERT mRNA in cadmium-transformed 16HBE cells.

Methods: Telomerase activity and expression of genes were measured after cultured cadmium-transformed 16HBE cells were exposed to sodium selenite at different doses (0.625, 1.25, 2.50, 5.00 micromol/L) for 24 hours.

Results: Selenium decreased telomerase activity in cadmium-transformed 16HBE cells. There existed an obvious dose-effect relationship between the selenium concentration and these changes. The expression of hTERT and c-myc mRNA also decreased but the expression of mad1 mRNA increased after exposure to selenium for 24 hours. No difference was found in expression of hTRF1 and hTRF2 mRNA after incubated with sodium selenite for 24 hours, compared with control group.

Conclusion: Selenium inhibits telomerase activity by decreasing hTERT and c-myc mRNA expression and increasing mad1 mRNA expression in cadmium-transformed 16HBE cells and selenium concentration is significantly correlated with these changes.

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硒对镉转化16HBE细胞端粒酶活性和hTERT表达的抑制作用。

目的:探讨亚硒酸钠对镉转化16HBE细胞端粒酶活性及hTERT mRNA表达的影响。方法:将培养的镉转化16HBE细胞以不同剂量(0.625、1.25、2.50、5.00微mol/L)亚硒酸钠处理24h，检测细胞端粒酶活性及基因表达。结果:硒降低镉转化16HBE细胞端粒酶活性。硒浓度与这些变化之间存在明显的剂量效应关系。硒处理24小时后，hTERT和c-myc mRNA表达降低，而mad1 mRNA表达升高。与对照组相比，亚硒酸钠孵育24小时后hTRF1和hTRF2 mRNA的表达无显著差异。结论:硒通过降低镉转化16HBE细胞中hTERT和c-myc mRNA的表达，增加mad1 mRNA的表达来抑制端粒酶活性，硒浓度与这些变化有显著相关性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biomedical and environmental sciences : BES

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