Xian Zhang, Xinsheng Cheng, Chuanqin Wang, Zheng Xi, Qiuhua Li
{"title":"Efficient High-Performance Liquid Chromatography with Liquid-Liquid Partition Cleanup Method for the Determination of Pymetrozine in Tobacco","authors":"Xian Zhang, Xinsheng Cheng, Chuanqin Wang, Zheng Xi, Qiuhua Li","doi":"10.1002/adic.200790015","DOIUrl":null,"url":null,"abstract":"<p>For the first time, a novel, simple and reliable method for analysis of pymetrozine residues in flue-cured tobacco leaves has been developed utilizing HPLC-UV with liquid-liquid partition cleanup. Pre-treatment with ultrasonic extraction and liquid-liquid partition procedures gave preferable baseline separation and clean chromatograms by removing water-soluble and fat-soluble components which interfere with pymetrozine in the test. The performance of the method was evaluated and validated: the detection limit (LOD) was 0.005μg.mL<sup>-1</sup>, the relative standard deviation (RSD) was 1.2 % (n=5), and the overall recovery was above 90 % at fortification levels of 0.200, 0.500, 1.000, and 5.000 mg.kg<sup>-1</sup>. The proposed method was successfully employed for the determination of pymetrozine residues in twelve flue-cured tobacco samples collected from different regions of China.</p>","PeriodicalId":8193,"journal":{"name":"Annali di chimica","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2007-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/adic.200790015","citationCount":"15","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annali di chimica","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/adic.200790015","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 15
Abstract
For the first time, a novel, simple and reliable method for analysis of pymetrozine residues in flue-cured tobacco leaves has been developed utilizing HPLC-UV with liquid-liquid partition cleanup. Pre-treatment with ultrasonic extraction and liquid-liquid partition procedures gave preferable baseline separation and clean chromatograms by removing water-soluble and fat-soluble components which interfere with pymetrozine in the test. The performance of the method was evaluated and validated: the detection limit (LOD) was 0.005μg.mL-1, the relative standard deviation (RSD) was 1.2 % (n=5), and the overall recovery was above 90 % at fortification levels of 0.200, 0.500, 1.000, and 5.000 mg.kg-1. The proposed method was successfully employed for the determination of pymetrozine residues in twelve flue-cured tobacco samples collected from different regions of China.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
