{"title":"An improved technique for counting leucocytes in milk by the direct microscopic method.","authors":"F H Newbould, L W Phipps","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>An improved microscopic method for counting leucocytes in milk has been developed, which results in acceptable levels of reproducibility. To overcome the lack of uniform cell distribution within a film a stratified sampling of the total film area is examined, which prevents the counting of any one area twice. The mean between-film coefficient of variation (S.D./x X 100) for all milk samples examined which contained more than 350,000 leucocytes/ml. was less than 10%.</p>","PeriodicalId":72497,"journal":{"name":"Canadian journal of comparative medicine and veterinary science","volume":"31 3","pages":"65-9"},"PeriodicalIF":0.0000,"publicationDate":"1967-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1494644/pdf/vetsci00004-0011.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Canadian journal of comparative medicine and veterinary science","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
An improved microscopic method for counting leucocytes in milk has been developed, which results in acceptable levels of reproducibility. To overcome the lack of uniform cell distribution within a film a stratified sampling of the total film area is examined, which prevents the counting of any one area twice. The mean between-film coefficient of variation (S.D./x X 100) for all milk samples examined which contained more than 350,000 leucocytes/ml. was less than 10%.