Mapping and Marker-assisted Selection of a Brown Planthopper Resistance Gene bph2 in Rice (Oryza sativa L.)

SUN Li-Hong , WANG Chun-Ming , SU Chang-Chao , LIU Yu-Qiang , ZHAI Hu-Qu , WAN Jian-Min
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引用次数: 61

Abstract

Nilaparvata lugens Stål (brown planthopper, BPH), is one of the major insect pests of rice (Oryza sativa L.) in the temperate rice-growing region. In this study, ASD7 harboring a BPH resistance gene bph2 was crossed to a susceptible cultivar C418, a japonica restorer line. BPH resistance was evaluated using 134 F2:3 lines derived from the cross between “ASD7” and “C418”. SSR assay and linkage analysis were carried out to detect bph2. As a result, the resistant gene bph2 in ASD7 was successfully mapped between RM7102 and RM463 on the long arm of chromosome 12, with distances of 7.6 cM and 7.2 cM, respectively. Meanwhile, both phenotypic selection and marker-assisted selection (MAS) were conducted in the BC1F1 and BC2F1 populations. Selection efficiencies of RM7102 and RM463 were determined to be 89.9% and 91.2%, respectively. It would be very beneficial for BPH resistance improvement by using MAS of this gene.

水稻抗褐飞虱基因bph2的定位与标记辅助选择
褐飞虱(Nilaparvata lugens stamatl)是温带水稻产区水稻(Oryza sativa L.)的主要害虫之一。本研究将携带bph2抗性基因的ASD7与粳稻恢复系易感品种C418杂交。利用“ASD7”与“C418”杂交的134个F2:3品系对BPH抗性进行了评价。对bph2进行SSR检测和连锁分析。结果,ASD7的抗性基因bph2成功定位在12号染色体长臂RM7102和RM463之间,距离分别为7.6 cM和7.2 cM。同时,对BC1F1和BC2F1群体进行表型选择和标记辅助选择(MAS)。RM7102和RM463的选择效率分别为89.9%和91.2%。利用该基因的MAS对BPH抗性的提高是非常有益的。
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