{"title":"Principles of a nanoscale DNA scanner.","authors":"E Y Chan","doi":"10.1049/ip-nbt:20041098","DOIUrl":null,"url":null,"abstract":"<p><p>A nanofluidic device for the routine stretching of single DNA molecules was hypothesised and tested. The device consists of an entrance channel leading to a post-field preceding an elongational flow field. The device facilitates each molecule's coil-to-stretch transition, counteracts its entropic recoil, and presents a stream of moving stretched molecules for detailed single-molecule time-of-flight measurements. The physics of DNA stretching was explored in a device where there was a juxtaposition of hexagonal upright post arrays with lithographically defined elongational flow fields.</p>","PeriodicalId":87402,"journal":{"name":"IEE proceedings. Nanobiotechnology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2004-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1049/ip-nbt:20041098","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"IEE proceedings. Nanobiotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1049/ip-nbt:20041098","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
A nanofluidic device for the routine stretching of single DNA molecules was hypothesised and tested. The device consists of an entrance channel leading to a post-field preceding an elongational flow field. The device facilitates each molecule's coil-to-stretch transition, counteracts its entropic recoil, and presents a stream of moving stretched molecules for detailed single-molecule time-of-flight measurements. The physics of DNA stretching was explored in a device where there was a juxtaposition of hexagonal upright post arrays with lithographically defined elongational flow fields.