[Estrogen receptor as a candidate gene for prolificacy of small tail Han sheep].

Xiao-Dan Bi, Ming-Xing Chu, Hai-Guo Jin, Li Fang, Su-Cheng Ye
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Abstract

Single nucleotide polymorphism in exon 1 of the estrogen receptor (ESR) gene was detected by PCR-SSCP in both high fecundity sheep breeds (Small Tail Han sheep, Hu sheep and German Mutton Merino sheep) and low fecundity sheep breeds (Dorset sheep,Suffolk sheep). Results indicated that there were three genotypes (AA, AB and BB) in all three high fecundity sheep breeds, but only two genotypes (AA, AB) in both low fecundity breeds. In Hu sheep,German Mutton Merino sheep, Small Tail Han sheep, Suffolk sheep and Dorset sheep,the frequency of allele A was 0.672, 0.786, 0.846, 0.857 and 0.867, respectively, and the frequency of allele B was 0.328, 0.214, 0.154, 0.143, and 0.133, respectively. Sequencing revealed a C-->G mutation at 363 bp of exon 1 of ESR gene in the BB genotype in comparison to the AA genotype. The genotype distribution was significantly different between Small Tail Han sheep and Hu sheep (P<0.01) and between Dorset sheep and Hu sheep (P <0.05). There was no difference in genotype distribution between other sheep breeds. The Small Tail Han sheep ewes with genotypes AB or BB had 0.51 (P < 0.05) and 0.7 (P < 0.05) more lambs than those with genotype AA, respectively. These results showed that the estrogen receptor locus is either a major gene that influences the prolificacy in Small Tail Han sheep or in close linkage with such a gene. In view of our results, marker-assisted selection using ESR is warranted to increase litter size in sheep and will be of considerable economic value to mutton producers.

[雌激素受体作为小尾寒羊繁殖能力的候选基因]。
用PCR-SSCP方法检测了高繁殖力绵羊品种(小尾寒羊、胡羊和德国羊肉美利奴羊)和低繁殖力绵羊品种(多赛特羊、萨福克羊)雌激素受体(ESR)基因外显子1单核苷酸多态性。结果表明,3个高繁殖力绵羊品种均存在3种基因型(AA、AB和BB),而2个低繁殖力绵羊品种均存在2种基因型(AA、AB)。在湖羊、德国羊肉美利奴羊、小尾寒羊、萨福克羊和多塞特羊中,等位基因A的频率分别为0.672、0.786、0.846、0.857和0.867,等位基因B的频率分别为0.328、0.214、0.154、0.143和0.133。与AA基因型相比,BB基因型的ESR基因外显子1 363 bp处出现C- >G突变。小尾寒羊和湖羊的基因型分布差异显著(P
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