Viruria of Human BK Virus and John Cunningham Virus among Renal Transplant Recipients and Healthy Control in Southeast of Caspian Sea.

IF 3.2 4区医学 Q3 VIROLOGY

Intervirology Pub Date : 2021-01-01 Epub Date: 2021-02-17 DOI:10.1159/000513369

Fereshteh Safaei, Alireza Mohebbi, Mina Hassanpour, Hadi Razavi Nikoo, Alijan Tabarraei

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引用次数: 4

Abstract

Background: Members of the Polyomaviridae family, BK virus (BKV), and John Cunningham virus (JCV) are linked to polyomavirus-associated nephropathy-associated transplant rejection in immunodeficient patients.

Objective: The aim of the study was to evaluate the prevalence of BKV and JCV in immunocompetent individuals in the north of Iran.

Methods: Ninety-one urine samples were obtained from renal transplant recipients with a mean age of 39.78 ± 11.19 years. A healthy control group of 65 volunteers with an average age of 40.32 ± 10.7 years also contributed. After DNA extraction, positive cases were detected through PCR. Genotyping was done by alignment and phylogenetic tree construction of the VP1 region against all known JCV and BKV genotypes.

Results: The prevalence of BKV and JCV was 15.38 and 19.78%, respectively. JCV was detected in 7.69% of the control group. The prevalence of the BKV between the case and control groups was significant (p < 0.0001). There was no significant association between BKV and JCV and duration of dialysis (p > 0.05). Overall, 62.16% of JCV cases were genotype I. Besides, genotype II was dominant within patients with BKV-positive patients.

Discussion: The results obtained here show a relatively lower prevalence of BKV and JCV in immunocompromised renal transplant receivers and healthy control than those reported from other areas in Iran. JCV genotyping was evaluated for the first time in Iran. Genotype I for JCV and genotype II for BKV were dominant genotypes in the north of Iran.

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里海东南部地区肾移植受者中人BK病毒和约翰·坎宁安病毒的病毒感染及健康对照。

背景:多瘤病毒科成员，BK病毒(BKV)和约翰坎宁安病毒(JCV)与免疫缺陷患者多瘤病毒相关肾病相关移植排斥反应有关。目的:本研究的目的是评估伊朗北部免疫正常人群中BKV和JCV的流行情况。方法:收集肾移植受者尿液91份，平均年龄39.78±11.19岁。健康对照组65名，平均年龄40.32±10.7岁。提取DNA后，PCR检测阳性病例。通过VP1区与所有已知的JCV和BKV基因型的比对和系统发育树构建进行基因分型。结果:BKV和JCV感染率分别为15.38%和19.78%。对照组JCV检出率为7.69%。病例组与对照组间BKV患病率差异有统计学意义(p < 0.0001)。BKV、JCV与透析时间无显著相关性(p > 0.05)。总体而言，基因i型占JCV病例的62.16%，bkv阳性患者中基因II型占主导地位。讨论:这里获得的结果显示，与伊朗其他地区的报告相比，免疫功能低下的肾移植受体和健康对照中BKV和JCV的患病率相对较低。在伊朗首次对JCV基因分型进行了评估。在伊朗北部，JCV的基因I型和BKV的基因II型是主要的基因型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Intervirology 医学-病毒学

CiteScore

5.40

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： ''Intervirology'' covers progress in both basic and clinical virus research, and aims to provide a forum for the various disciplines within virology. Issues publishing original papers alternate with thematic issues, focusing on clearly defined topics. This thematic concentration serves to make timely reviews, research reports and controversy easily accessible to both specialists in the field and those who want to keep track of the latest developments outside their own area of interest. In addition to original papers, regular issues publish short communications and letters to the editor to provide readers with a forum for the exchange of ideas and comments. The scope encompasses work on the molecular biology of human and animal viruses, including genome organization and regulation, and the structure and function of viral proteins. The pathogenesis, immunology, diagnosis, epidemiology, prophylaxis and therapy of viral diseases are considered.