Development of rapid immunochromatographic test with recombinant NcSAG1 for detection of antibodies to Neospora caninum in cattle.

Clinical and diagnostic laboratory immunology Pub Date : 2005-07-01 DOI:10.1128/CDLI.12.7.885-887.2005

Min Liao, Shoufa Zhang, Xuenan Xuan, Guohong Zhang, Xiaohong Huang, Ikuo Igarashi, Kozo Fujisaki

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引用次数: 23

Abstract

An immunochromatographic test (ICT) with recombinant surface antigen 1 of Neospora caninum (NcSAG1) was developed for the rapid detection of antibodies to N. caninum in cattle. The ICT was used to clearly discriminate between immunofluorescent-antibody test (IFAT)-positive bovine sera and IFAT-negative bovine sera. Serum samples collected from cattle in Yanbian, China, were examined by the ICT. Of the 96 serum samples, 23 (24.0%) were positive by the ICT, and 19 (19.8%) samples were positive by a previously developed enzyme-linked immunosorbent assay (ELISA). Eighteen of 19 ELISA-positive samples were positive according to the ICT. A good agreement was found between the results of the ICT and the ELISA. The results presented here suggest that the ICT with recombinant truncated NcSAG1 fused to glutathione S-transferase is a useful and reliable method for the detection of antibodies to N. caninum in cattle.

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重组NcSAG1快速免疫层析检测牛新孢子虫抗体的建立。

建立了重组犬新孢子虫表面抗原1 (NcSAG1)免疫层析法(ICT)快速检测牛新孢子虫抗体的方法。ICT被用来明确区分免疫荧光抗体试验(IFAT)阳性牛血清和IFAT阴性牛血清。ICT对从中国延边采集的牛血清样本进行了检测。96份血清样本中，ICT检测阳性23份(24.0%)，ELISA检测阳性19份(19.8%)。19份elisa阳性样品中18份经ICT检测呈阳性。ICT和ELISA的结果非常吻合。本研究结果表明，重组截断的NcSAG1与谷胱甘肽s -转移酶融合的信息通信技术(ICT)是一种有效、可靠的检测牛链球菌抗体的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Clinical and diagnostic laboratory immunology

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