The fine structure of inhibitory synapses in the crayfish.

R P PETERSON, F PEPE
{"title":"The fine structure of inhibitory synapses in the crayfish.","authors":"R P PETERSON,&nbsp;F PEPE","doi":"10.1083/jcb.11.1.157","DOIUrl":null,"url":null,"abstract":"<p><p>Physiological investigations have shown that the synaptic input to the sensory neuron of the stretch receptor in the abdominal muscles of the crayfish is purely inhibitory. This neuron was chosen, therefore, as a site in which to study the fine structure of inhibitory synaptic endings. It was hoped that this fine structure might (a) provide a morphological prototype for the study of more complex synaptic systems and (b) reflect the inhibitory mechanisms. Stretch receptors were fixed in situ in buffered OsO(4), dehydrated, and embedded in Araldite. Both cross and longitudinal sections were examined after staining with phosphotungstic acid. The inhibitory endings were easily identified by their great similarity to previously described excitatory endings. Small circular profiles (synaptic vesicles) about 460 A in diameter and an accumulation of mitochondria were consistently observed within the presynaptic endings. An increased osmiophilia of pre- and postsynaptic membranes, where they were in apposition, was also seen. The only observed difference between these inhibitory endings and excitatory endings, described by other authors, was the variable presence of a latticework of 230 A tubules in the connective tissue immediately adjacent to the inhibitory endings. Inhibitory endings were observed on all parts of the sensory neuron except the axon.</p>","PeriodicalId":22618,"journal":{"name":"The Journal of Biophysical and Biochemical Cytology","volume":"11 ","pages":"157-69"},"PeriodicalIF":0.0000,"publicationDate":"1961-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1083/jcb.11.1.157","citationCount":"47","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Journal of Biophysical and Biochemical Cytology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1083/jcb.11.1.157","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 47

Abstract

Physiological investigations have shown that the synaptic input to the sensory neuron of the stretch receptor in the abdominal muscles of the crayfish is purely inhibitory. This neuron was chosen, therefore, as a site in which to study the fine structure of inhibitory synaptic endings. It was hoped that this fine structure might (a) provide a morphological prototype for the study of more complex synaptic systems and (b) reflect the inhibitory mechanisms. Stretch receptors were fixed in situ in buffered OsO(4), dehydrated, and embedded in Araldite. Both cross and longitudinal sections were examined after staining with phosphotungstic acid. The inhibitory endings were easily identified by their great similarity to previously described excitatory endings. Small circular profiles (synaptic vesicles) about 460 A in diameter and an accumulation of mitochondria were consistently observed within the presynaptic endings. An increased osmiophilia of pre- and postsynaptic membranes, where they were in apposition, was also seen. The only observed difference between these inhibitory endings and excitatory endings, described by other authors, was the variable presence of a latticework of 230 A tubules in the connective tissue immediately adjacent to the inhibitory endings. Inhibitory endings were observed on all parts of the sensory neuron except the axon.

小龙虾抑制性突触的精细结构。
生理学研究表明,对小龙虾腹肌拉伸受体感觉神经元的突触输入是纯抑制性的。因此,这个神经元被选为研究抑制性突触末梢精细结构的地点。希望这种精细结构可以(a)为研究更复杂的突触系统提供形态学原型,(b)反映抑制机制。拉伸受体原位固定在缓冲的OsO(4)中,脱水后包埋在Araldite中。磷钨酸染色后,观察横切面和纵切面。抑制性末梢与先前描述的兴奋性末梢非常相似,因此很容易识别。在突触前末端一致观察到直径约460 A的小圆形轮廓(突触囊泡)和线粒体的积累。突触前膜和突触后膜的亲锇性增加,突触前膜和突触后膜处于相对位置。其他作者所描述的这些抑制性末梢和兴奋性末梢之间唯一观察到的区别是,在紧邻抑制性末梢的结缔组织中,存在可变的230a小管晶格。除轴突外,感觉神经元各部位均可见抑制性末梢。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信