Design, synthesis and properties of hydrogen peroxide fluorescent probe based on benzothiazole

IF 4.5 2区医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioorganic Chemistry Pub Date : 2022-06-01 DOI:10.1016/j.bioorg.2022.105798

Tao Ma, Ye Zhang, Kai Fu, Zhichun Li, Changchun Yuan, Wenbing Ma

引用次数: 7

Abstract

Abnormal intracellular hydrogen peroxide (H₂O₂) concentration is related to cancer, cardiovascular, cerebrovascular diseases and other diseases. A novel H₂O₂ fluorescent probe (BBS) was designed and synthesized. The fluorescence intensity shows good linear relationship with the concentration of H₂O₂ (0–40 μM), and the detection limit is 132 nM. The response mechanism of probe BBS was verified and analyzed by UV–Vis spectra, titration spectra and density functional theory (DFT) on Gaussian 09 programme. BBS can be used for bioimaging in living cells.

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基于苯并噻唑的过氧化氢荧光探针的设计、合成及性能研究

细胞内过氧化氢(H2O2)浓度异常与癌症、心脑血管疾病等疾病有关。设计并合成了一种新型H2O2荧光探针。荧光强度与H2O2浓度(0 ~ 40 μM)呈良好的线性关系，检出限为132 nM。在高斯09程序上，通过紫外可见光谱、滴定光谱和密度泛函理论(DFT)验证并分析了探针BBS的响应机理。BBS可用于活细胞的生物成像。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioorganic Chemistry 生物-生化与分子生物学

CiteScore

9.70

自引率

3.90%

发文量

679

审稿时长

31 days

期刊介绍： Bioorganic Chemistry publishes research that addresses biological questions at the molecular level, using organic chemistry and principles of physical organic chemistry. The scope of the journal covers a range of topics at the organic chemistry-biology interface, including: enzyme catalysis, biotransformation and enzyme inhibition; nucleic acids chemistry; medicinal chemistry; natural product chemistry, natural product synthesis and natural product biosynthesis; antimicrobial agents; lipid and peptide chemistry; biophysical chemistry; biological probes; bio-orthogonal chemistry and biomimetic chemistry. For manuscripts dealing with synthetic bioactive compounds, the Journal requires that the molecular target of the compounds described must be known, and must be demonstrated experimentally in the manuscript. For studies involving natural products, if the molecular target is unknown, some data beyond simple cell-based toxicity studies to provide insight into the mechanism of action is required. Studies supported by molecular docking are welcome, but must be supported by experimental data. The Journal does not consider manuscripts that are purely theoretical or computational in nature. The Journal publishes regular articles, short communications and reviews. Reviews are normally invited by Editors or Editorial Board members. Authors of unsolicited reviews should first contact an Editor or Editorial Board member to determine whether the proposed article is within the scope of the Journal.