{"title":"A novel family of retrotransposons in Xenopus with a developmentally regulated expression.","authors":"S Shim, S K Lee, J K Han","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Summary: </strong>We have obtained a novel family of LTR-retrotransposons in Xenopus laevis, named Xretpos, from cDNA and genomic clones. Its long terminal repeats (LTRs) can be subdivided into U3, R, and U3 to U5 region, and are bounded by 6 bp inverted repeats. Xretpos contains primer binding site and polypurine tract, and multiple copies of Xretpos-related element are present in the genome. A long open reading frame (ORF) encodes the CCHC motif conserved in retroviral gag proteins and leucine zipper motif capable of forming the coiled-coil. However, no amino acid homology to usually conserved retroviral pol gene was revealed. We report that in vitro synthesized Xretpos complementary RNAs are translated to produce a predicted size of protein. We also show that zygotically activated Xretpos transcripts are restricted to ventro-posterior specific regions and induced by UV-irradiation and BMP-4 overexpression in cycloheximide-dependent way. genesis 26:198-207, 2000.</p>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"99","ListUrlMain":"","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Summary: We have obtained a novel family of LTR-retrotransposons in Xenopus laevis, named Xretpos, from cDNA and genomic clones. Its long terminal repeats (LTRs) can be subdivided into U3, R, and U3 to U5 region, and are bounded by 6 bp inverted repeats. Xretpos contains primer binding site and polypurine tract, and multiple copies of Xretpos-related element are present in the genome. A long open reading frame (ORF) encodes the CCHC motif conserved in retroviral gag proteins and leucine zipper motif capable of forming the coiled-coil. However, no amino acid homology to usually conserved retroviral pol gene was revealed. We report that in vitro synthesized Xretpos complementary RNAs are translated to produce a predicted size of protein. We also show that zygotically activated Xretpos transcripts are restricted to ventro-posterior specific regions and induced by UV-irradiation and BMP-4 overexpression in cycloheximide-dependent way. genesis 26:198-207, 2000.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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