Tissue distribution of recombinant human tumor necrosis factor alpha derivative in mice.

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-04-01

B X Chai, Z M Tang, X W Liu, M Tu

{"title":"Tissue distribution of recombinant human tumor necrosis factor alpha derivative in mice.","authors":"B X Chai, Z M Tang, X W Liu, M Tu","doi":"","DOIUrl":null,"url":null,"abstract":"Aim: To study the tissue distribution and its mechanism of a new recombinant tumor necrosis factor alpha derivative (rhTNF alpha Da) in mice.Methods: 125I-rhTNF alpha Da was prepared by Iodogen method. Tissue distribution of 125I-rhTNF alpha Da in mice was studied by determining radioactivity of tetrachloroacetic acid (TCA)- precipitable fraction in tissues. The isolated heart-lung perfusion study using 125I-rhTNF alpha Da perfusate was carried out to study the distribution characteristics of 125I-rhTNF alpha Da in lung.Results: Except for thyroid, AUC of the TCA-precipitable 125I-rhTNF alpha Da in tissues was highest in lung, which was 12.2-fold of that in serum, while concentrations in other tissues were all lower than that in serum. Perfusion study in vitro revealed that the concentration of radio-labeled peptide in lung was higher than that in perfusate. On the contrary, level in heart was much lower than that in perfusate. The overall distribution of 125I-rhTNF alpha Da in lungs showed rapidly equilibratory, dose-dependent, saturable, competitive, and highly affinitive, with Kd 47.6 pmol.L-1 and Bmax 348 fmol.g-1 (lung tissue).Conclusion: The specific distribution of rhTNF alpha Da in lungs was its distinctive characteristics.","PeriodicalId":24002,"journal":{"name":"Zhongguo yao li xue bao = Acta pharmacologica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zhongguo yao li xue bao = Acta pharmacologica Sinica","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Aim: To study the tissue distribution and its mechanism of a new recombinant tumor necrosis factor alpha derivative (rhTNF alpha Da) in mice.

Methods: 125I-rhTNF alpha Da was prepared by Iodogen method. Tissue distribution of 125I-rhTNF alpha Da in mice was studied by determining radioactivity of tetrachloroacetic acid (TCA)- precipitable fraction in tissues. The isolated heart-lung perfusion study using 125I-rhTNF alpha Da perfusate was carried out to study the distribution characteristics of 125I-rhTNF alpha Da in lung.

Results: Except for thyroid, AUC of the TCA-precipitable 125I-rhTNF alpha Da in tissues was highest in lung, which was 12.2-fold of that in serum, while concentrations in other tissues were all lower than that in serum. Perfusion study in vitro revealed that the concentration of radio-labeled peptide in lung was higher than that in perfusate. On the contrary, level in heart was much lower than that in perfusate. The overall distribution of 125I-rhTNF alpha Da in lungs showed rapidly equilibratory, dose-dependent, saturable, competitive, and highly affinitive, with Kd 47.6 pmol.L-1 and Bmax 348 fmol.g-1 (lung tissue).

Conclusion: The specific distribution of rhTNF alpha Da in lungs was its distinctive characteristics.

本刊更多论文

重组人肿瘤坏死因子α衍生物在小鼠体内的组织分布。

目的:研究一种新的重组肿瘤坏死因子α衍生物(rhTNF α Da)在小鼠体内的组织分布及其作用机制。方法:采用碘法制备125I-rhTNF α - Da。采用四氯乙酸(TCA)可沉淀组分放射性测定法，研究了125I-rhTNF α - Da在小鼠组织中的分布。采用125I-rhTNF - α - Da灌注液进行离体心肺灌注研究，研究125I-rhTNF - α - Da在肺中的分布特征。结果:tca可沉淀125I-rhTNF α Da在各组织中的AUC除甲状腺外以肺最高，为血清AUC的12.2倍，其他组织均低于血清AUC。体外灌注研究显示肺内放射性标记肽浓度高于灌注液。相反，心脏中的含量远低于灌注液中的含量。125I-rhTNF α - Da在肺中的总体分布表现为快速平衡、剂量依赖性、饱和、竞争性和高度亲和性，Kd值为47.6 pmol。L-1和Bmax为348 fmol。G-1(肺组织)。结论:rhTNF α - Da在肺组织的特异性分布具有明显的特点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Zhongguo yao li xue bao = Acta pharmacologica Sinica

自引率

0.00%

发文量