[Metabolism of methionine in tissues of cattle in vitro].

Abstract

After incubation of homogenates of liver, kidney, brain, skeletal muscles mucosa of rumen and empty intestine of cattle in Krebs-Ringer's phosphate buffer with [2-14C]methionine 25.8-43.4% radioactive label was found in protein; 28.1-42.0%, in CO2; 12.0-18.6%, in glucose; 8.2-14.9%, in glycogen; 3.1-6.3%, in lipids. The highest level of [2-14C]methionine utilization in protein synthesis was found in skeletal muscle; in energetical processes, in liver; in the process of gluconeogenesis, in skeletal muscles and liver; in lipid synthesis, in brain; the lowest level of [2-14C]methionine utilisation in above-mentioned process was found in skin.